Antisense oligonecleotides of CT120 inhibit the growth of lung adenocarcinoma cell line A549 / 基础医学与临床

Objective To clarify the relationship between CT120,a novel human plasma membrane-associated gene,and the proliferation of lung adenocarcinoma cell line A549.Methods Expression vector(pcDNA3.1)containing antisense oligonucleotides of CT120 was constructed and transfected into the adenocarcinoma cell line A549.RT-PCR and Western blot detected the expression of CT120.Meantime,flow cytometry and soft agarose colony formation were applied for cell proliferation,and P53,CyclinD1 and CDK4 were detected by RT-PCR.ResultspcDNA3.1 containing antisense oligonucleotides of CT120 was successfully constructed and inhibited the expression of CT120 effectively by RT-PCR and Western blot.The expression of P53 was up-regulated and the expression of CyclinD1 and CDK4 were down-regulated.Conclusion The down-regulation of CT120 expression by antisense oligonucleotides technique may be a potential drug target for treatment of lung cancer.

Practice on applied psychological laboratory construction and exploration on experimental teaching mode / 中华医学教育探索杂志

The experimental teaching is one of the important parts of psychology teaching.How to make the best of the lab resource to efficient experiment teaching is an important topic on psychological specialty construction.We have explored the construction of psychological lab and experiment teaching,and provided some helpful guidance for others.