Chemical Constituents of Endophytic Fungus Aconitum Transsectum from Alternaria Solani / 中国药师

Objective: To study the chemical constituents of endophytic fungus Alternaria solani from Aconitum transsectum. Methods: The compounds were isolated and purified by column chromatography , and their structures were elucidated based on the spectroscopic analysis (1H-NMR, 13C-NMR and MS).Results:Totally 15 compounds were isolated from A.solani and characterized as 20-hydroxyl-ergosta-4,6,8(14),22-tetraen-3-one(1),ergone(2),stellasterin(3),episterol(4),ergosterol peroxide(5),cyclo(D)-Pro-(D)-Leu(6),cyclo-(L-Val-L-Pro)(7),cyclo-(S-Pro-S-Ile)(8),tentoxin(9),porrotoxin(10),7-dehydroxyl-zinniol(11),zinniol (12),8-zinniol methyl ether (13),3-methoxy-4-methyl-5-(3′,3′-dimethylallyloxy)-2-methoxymethyl benzoic acid (14) and pseudo-monas aeruginosa H2-sesquiterpene lactone (15).Conclusion: Totally 15 compounds are isolated from endophytic fungus Alternaria solani, and compounds 2-10 and 12-15 are isolated for the first time .

Studies on Diterpenoid Alkaloids in Aconitum Transsectum / 中国药师

Objective:To study the chemical constituents in the roots of Aconitum transsectum. Methods:The compounds were i-solated and purified by column chromatography on the columns of silica gel, Al2 O3 and Sephadex LH-20, and their structures were elu-cidated on the basis of spectroscopic evidence ( MS and NMR) . Results:Totally 12 compounds were isolated from A. transsectum, and characterized as yunaconitine (1), indaconitine (2), crassialine A (3), 8-deacetylyunaconitine (4), talatisamine (5), chasma-conitine (6), 14-acetyltalatisamine (7), vilmoraconitine (8), sachaconitine (9), 14-acetylsachaconitine (10), acoforestinine (11) and vilmorrianine C (12). Conclusion:Compounds 8-11 are obtained from A. transsectum for the first time.

Study on Diterpenoid Alkaloids from the Roots of Aconitum Straminiflorum / 中国药师

Objective:To study the chemical constituents of Aconitum straminiflorum. Methods:The air-dried roots of A. stra-miniflorum were powdered and extracted three times with 90% ethonal under reflux. After the removal of the solvent under reduced pressure,the crude extract was dissolved in 1. 5% HCl solution(7 L). The acidic solution was basified to pH 9. 0 with ammonia (25%)and extracted with CHCl3 to obtain crude extract of total alkaloids after the removal of CHCl3 . The compounds were isolated and purified by column chromatography and identified based on spectral analysis( MS,1 H-üMR and 13 C-üMR). Results:Totally 7 compounds were isolated from A. straminiflorum,and characterized as aconosine(1),13-deoxyludaconitine(2),dolaconine(3), ezochasmanine(4),talatisamine(5),scopaline(6)and cammaconine(7),respectively. Conclusion:Seven diterpenoid alkaloids are isolated from A. Straminiflorum for the first time.