RESUMO
<p><b>OBJECTIVE</b>To study the direct effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury and elucidate their therapeutic mechanism on ischemic heart diseases.</p><p><b>METHOD</b>Cultured cardiomyocytes of neonatal rats were subjected to anoxia-reoxia injury which simulated the ischemia-reperfusion injury in vivo, and hawthorn leaf procyanidins were applied. The therapeutic effect was valued by LDH leakage and MTT test. For a further mechanism study, contents of MDA and activities of SOD in cardiomyocytes were measured.</p><p><b>RESULT</b>Hawthorn leaf procyanidins in 24-60 mg x L(-1) significantly and dose-dependently inhibited LDH leakage (compared with the model group, all P<0.01 ) and cell viability decrease (compared with model group, 24-48 mg x L(-1) groups all P<0.05; 60 mg x L(-1) group, P<0.01) in cardiomyocytes induced by anoxia-reoxia injury. Furthermore, hawthorn leaf procyanidins in 24-60 mg x L(-1) significantly inhibited the increase of MDA content (compared with the model group, all P<0.01) and the decreased of SOD activity (compared with the model group, 24 mg x L(-1) group, P<0.05; other groups all P<0.01) in cardiomyocytes undergoing anoxia-reoxia injury.</p><p><b>CONCLUSION</b>Hawthorn leaf procyanidins have a significant therapeutic effect on the simulated ischemia-reperfusion injury of cultured neonatal rat cardiomyocytes, which may relate to their anti-oxidation effects. And the direct protective effect of hawthorn leaf procyanidins on cardiomyocytes subjected to ischemia-reperfusion injury may be one of the key mechanisms of its therapeutic effect on ischemic heart diseases.</p>
Assuntos
Animais , Feminino , Ratos , Biflavonoides , Farmacologia , Catequina , Farmacologia , Sobrevivência Celular , Crataegus , Química , Hipóxia , Metabolismo , Patologia , L-Lactato Desidrogenase , Metabolismo , Malondialdeído , Metabolismo , Miócitos Cardíacos , Metabolismo , Folhas de Planta , Química , Proantocianidinas , Farmacologia , Traumatismo por Reperfusão , Metabolismo , Patologia , Superóxido Dismutase , MetabolismoRESUMO
Brain slice technique has been widely applied in the field of neuroscience.This article reviews the application of brain slice in anti-cerebral ischemia pharmacology research on electrophysiology,synaptic plasticity,pathomorphology,neurotransmitters and in the field of Chinese medicine.