Pharmacognostical, Pharmaceutical and Microbiological Analysis of Mrudwika Sharkara

Draksha (Mrudwika) is best among all fruits as per Ayurveda. It is also indicated in different diseases as a medication and used in diets in fresh and dry forms. Draksha is a fruit that is only available during certain times of the year. Sharkara dosage form of it can be prepared to make that is palatable. Draksha is used in dry form i.e., Munakka (Mrudwika, raisins). Commonly, Munakka, Kismis, and Currants are three varieties with slight differences in their characteristics and nutritional content. They own property in Mridu Rechana, Vatahara, Pittahara, Brimhana, Vrishya, and Rasayana. For health promotion, disease prevention, and a wide range of Vata, Pitta, and Raktaja Vyadhis and Apatarpana Vyadhis, Draksha should be regularly incorporated into diet in either dry or fresh form. In the present study, an attempt has been made to develop pharmacognostical and pharmaceutical standards for Mrudwika Sharkara for assurance of quality of herbal compounds pharmacognostical and pharmaceutical analysis should be done. Methods: Mrudwika Sharkara was subjected to microscopic evaluation for pharmacognostical study, analysis physic-chemical analysis includes specific gravity, pH value, reducing sugar, non-reducing sugar and total sugar and high Performance thin layer chromatography (HPTLC). Mrudwika Sharkara was assessed for microbiological which include smear examination and culture study. Results: Pharmacognostical study showed the presence of certain identifying characters of Mrudwika and Sharkara. In pharmaceutical study, preliminary physico-chemical analysis showed specific gravity is 1.160, pH value is 4, reducing sugar 21.1%, non reducing sugar 48%, total sugar content 69.1%. HPTLC analysis showed eight spots in 254nm and six spots in 366nm. From date of preparation 23/06/21 to 18/10/22 no fungal contamination was found in Mrudwika Sharkar Conclusion: Present work was carried out to standardize the formulation Mrudwika Sharkara in terms of its identity, quality and purity. All of the preparation's active ingredients were identified by pharmacognostic and physicochemical examination. Self life of Mrudwika Sharkara showed that the quality of syrup in standard condition.

PHYSICOCHEMICAL AND HPTLC ANALYSIS OF PIPPALIMULA (ROOT OF Piper longum LINN.)

Pippalimula (root of Piper longum Linn.) is the spice of Indian cuisine having immense therapeutic properties. The study was conducted to evaluate Physicochemical, phytochemical analysis and HPTLC analysis using specific solvent system for Pippalimula. Material and Methods: Fine powder of Grade I Pippalimula (root of Piper longum Linn.) and alchoholic extract were obtained and subjected to phytochemical analysis and chromatographic study. Discussion: Physicochemical analysis of the root was carried out. HPTLC analysis of the methanolic extract of the root powder was performed with Toluene: Ethyl acitate 9:1.(v/v) Conclusion: HPTLC analysis of root of Piper longum Linn.can provide standard analysis with selected solvent system and can be used as a reference for the authentication and quality control of the drug. The study will provide referential information for the correct identification of the crude drugs.

Comparative pharmacognostical & pharmaceutical evaluation of Vyaghri haritaki avaleha - an ayurvedic formulation .

Kasa is well-defined clinical condition in Brihadtrayi, clearly correlate with cough and its pathophysiology exactly correlates with the mechanism of cough reflex in contemporary medical science. Everywhere, Paediatric Outdoor Patients Department (OPD) has more than half of the total patients having respiratory tract complaints. So, to treat the disease Kasa, Vyaghri Haritaki Avaleha (VHA) was taken from Bhaishajya Ratnavali. The present work was carried out to standardize the raw drugs & finished product-VHA with comparing API standards and previous research work done in same institute to conform its identity, quality and purity of the final product. The pharmacognostical work reveals that presence of Epidermis, Cork, Pericycle, Phloem etc. from Kantakari; Lignified fibre, Stone cells etc. of Haritaki; Aluerone grains, Prismatic crystals etc. of Shunthi observed microscopically. Organoleptic features of VHA made out of the crude drugs were within the standard range. The pH value of VHA was 4.5, Water soluble extract was 71.9 %w/w, Loss on drying was 30.41 %w/w, Reducing sugar was 27.92 %w/w and High Performance Thin Layer Chromatography (HPTLC) at 254nm and 366nm resulted into 2 & 5 spots respectively

Pharmacognostical and Pharmacochemical Parameters of Tablet Sutashekhara Rasa – Without Gold .

Tablet Sutashekhara Rasa (TSR) is an Ayurvedic, herbo-metal formulation prescribed widely for several conditions such as Acid peptic disorders, Pain in abdomen, Haemorrhage, Mental disorders etc. On analysis of pharmcodynamics of this compound it is basically Pitta corrective drug. Ardhavabhedaka (Migraine) is also one of the clinical morbidity which is manifested by vitiated Pitta/Rakta along with Vata. The available treatment in modern medicine is use of NSAIDs, Beta-blockers etc. with only temporary relief. TSR being a Pitta corrective is used in a clinical study with new indication in Ardhavabhedaka (Migraine). Till date there is no data available regarding evaluation ofTSR. Present study an attempt to develop newer approaches for the quality control and standardization of TSR. The samples were subjected to organoleptic, physicochemical analysis and Chromatographic (HPTLC) examination by optimizing the solvent systems. The phrmacognostical study of ingredients of TSR shows the presence of Sceleriform vessel, Lignified stone cells, Bottle necked shapedstone cells etc. Pharmaceutical analysis showed that the Average weight of tablet 276mg, Average hardness of tablet 2.05 Kg/cm2 , Loss on drying 4.7904% w/w, pH value 7 and High Performance Thin Layer Chromatography at 254nm and 366nm resulted into 6 spots