Effects of transcutaneous electrical acupoint stimulation assisting sodium nitroprusside controlled hypotension on Glu,ATⅡ and SOD / 重庆医学

Objective To observe the effects of transdermal acupoint electric stimulation(TEAS)assisting sodium nitroprus-side induced controlled hypotension on serum glucose (Glu),angiotensin Ⅱ (ATⅡ)and superoxide dismutase (SOD),and to inves-tigate the protective effect of TEAS under controlled hypotension anesthesia.Methods 60 cases undergoing elective endoscopy si-nus surgery by adopting sodium nitroprusside induced controlled hypotension under general anaesthesia maintained the mean arterial pressure(MAP)in 50-60 mm Hg and were randomly and equally divided into two groups.The group Ⅰ conducted TEAS,while the group Ⅱ did not conduct TEAS.The controlled hypotension time and surgery time were recorded in the two groups;Glu and ATⅡ values were detected before anesthesia (T0 ),30 min after hypotension (T1 ),hypotension stopping(T2 );SOD was detected at T0 ,T2 ,30 min after hypotension(T3 ).Results The operation time and controlled hypotension continuous time had no statistically significant difference between the two groups(P >0.05).The Glu value in the group Ⅰ had no statistically significant difference a-mong the 3 time points,while which at T1 ,T2 was higher than that at T0 in the group Ⅱ(P 0.05),which at T1 ,T2 in the group Ⅱwas higher than that in the group Ⅰ (P <0.05);the SOD value at T2 was lower than that at T0 in the group Ⅰ,which at T2 ,T3 in the group Ⅱwas lower than that in the group Ⅰ(P <0.05).Conclusion TEAS assisting sodium nitroprusside controlled hypotension can better in-hibit the stress response.

Effect of protein kinase C in oocyte maturation, fertilization and preimplantation embryonic development / 生物医学工程学杂志

Protein kinase C (PKC) family plays a critical role in many developmental events, including oocyte activation, completion of the second meiosis and initiation of the first mitosis, compaction, and blastocysts formation as well. But little is known of its many isozymes. Studies have shown that 10 isozymes of PKC and its anchor protein, RACK, are expressed in the course from 2 cell stage through blastocyst stage in mouse. We reviewed here the recent studies on the location pattern and expression levels of different PKC isozymes. Those studies indicated that the isozymes were very important for every stage of preimplantation embryonic development, especially at the early 4-cell stage. Some are increased temporarily in nucleus, which indicated that they might control and regulate the remolding of embryonic nucleus. We also analyzed the possible functions of PKCs in the somatic nuclear transferred embryos.