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Objective To evaluate the efficiency and safety of micropulse laser trabeculoplasty (MLT) for open angle glaucoma ( OAG) patients and ocular hypertension ( OHT) patients. Methods A prospective self-controlled study was designed. Fifty-six eyes of 56 OAG and OHT patients in Peking University Third Hospital were recruited from June 2016 to June 2017,including 32 males and 24 females with the age of (50. 4±19. 0) years old. The diagnosis included 48 eyes of primary open angle glaucoma (POAG),3 eyes of normal tension glaucoma and 5 eyes of OHT. The best corrected vision acuity ( BCVA) was 0. 7 ±0. 3 before treatment. The intraocular pressure (IOP) before MLT was (20.4±5.8) mmHg,the average types of antiglaucoma medications was 1.7,including systemic medication and local drug use. The general information, BCVA, IOP and antiglaucoma medication were recorded. Only one eye of each patient was treated by MLT. The IOP at different follow-up time points (2 hours, 1 day,1 week, 1 month, 3 months post-treatment) and the number of antiglaucoma medication before and after treatment were compared. This study protocol was approved by Ethic Committee of Peking University Third Hospital (No. 2014166). Written informed consent was obtained from each subject prior to any medical examination. Results The IOP after MLT was (20. 2±6. 7),(17. 6±4. 4),(18. 1±4. 5),(17. 4±3. 4) and (17. 0±2. 1)mmHg at 2 hours, 1 day,1 week,1 month and 3 months,respectively,with significant difference among the different time points ( F=7. 320,P<0. 001). Compared with the IOP before treatment,the IOP at 1 day,1 week,1 month and 3 months after treatment were significantly decerased,with significant differences between them (all at P<0. 05). Three months after treatment,the average number of glaucoma medications used was 1. 5, and only local administration was used. Infection,hyphema,cornea injury,burns and other complications were not seen in all the patients. Conclusions MLT is effective and safety for OAG and OHT patients,and reduce the use of glaucoma drugs.
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BACKGROUND: Studies have demonstrated that platelet-rich plasma can promote osteogenesis and proliferation of bone marrow mesenchymal stem cells, adipose-derived stem cells and skeletal muscle satellite cell, but whether it can promote the proliferation and osteogenesis of human umbilical cord mesenchymal stem cells (hUC-MSCs) is unclear. OBJECTIVE: To investigate the effects of different concentrations of platelet-rich plasma on the proliferation and osteogenic differentiation of hUC-MSCs. METHODS: Passage 5 hUC-MSCs were cultured in medium containing different concentrations of platelet-rich plasma (0, 250, 500, 750, 1000, 2000 ng/L), respectively. Cell proliferation was detected at 1, 3, 5, 7 days after culture, and the best platelet-rich plasma mass concentration was screened. Afterwards, passage 5 hUC-MSCs were divided and cultured in complete medium (blank control group), optimal concentration of platelet-rich plasma (platelet rich plasma group), osteogenesis induction medium (osteogenic induction group), or the osteogenesis induction medium containing the optimal concentration of platelet-rich plasma (combined group). Cell activity of alkaline phosphatase was detected after cultivated for 3, 7, 14 days. Osteopontin, bone specific transcription factor, and osteocalcin mRNA relative expression levels were detected after cultivated for 7, 14, 21 days. Mineralization of the extracellular matrix was detected after cultivated for 21 days. RESULTS AND CONCLUSION: After 5 and 7 days of culture, the cell proliferation was higher in 500, 750, 1000 ng/L platelet-rich plasma groups than 0 ng/L group (P < 0.05), and 750 ng/L platelet-rich plasma showed the best effect on cell proliferation, which was used in the following experiments. Compared with the other groups, the combined group had significantly increased alkaline phosphatase activity (P < 0.05), and up-regulated osteopontin, bone specific transcription factor and osteocalcin mRNA relative expression levels (P < 0.05) at different culture times. In addition, the degree of extracellular matrix mineralization in the combined group was also higher than that in the other three groups (P < 0.05).To conclude, 750 ng/L platelet-rich plasma can promote hUC-MSCs proliferation and osteogenic differentiation.
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Objective To explore the effect of the H-uvulopalatopharyngology(H-UPPP)on the obstructive sleep apnea hypopnea syndrome(OSAHS)with hypertension sufferers.Methods The clinical data of 100 cases of OSAHS patients with hypertension were analyzed retrospectively,and the related parameters about changes of blood pressure and respiration were analyzed.Results The sargical operation treatment cure rate of OSAHS eas 37%,and the effective rate was 81%.In those patients.all the hypertension patients who in the first stage were completely cured (46/46),while for whom in the second stage,the cure rate was 37%(13/35).So the total effective rate of experimental group Was 88%(30/35).19 cases was ineffective.In those 19 patients,there were 16 patients in the second stage,while 3 cases in the third stage.Conclusion H-UPPP could completely cure OSAHS,meanwhile,it also obviously lowered hypertension which induced by OSAHS,and made the sufferers keep at relative normal blood pressure for a long time.So H-UPPP Was a best treatment method to the OSAHS patients with hypertension.