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Objective To evaluate the clinical and histological features of patients with abnormal liver tests of unknown etiology, and then to investigate the diagnosis and differential diagnosis. Methods Patients with abnormal liver function test hospitalized and had liver biopsies during 2008-2009 constituted this retrospective study cohort. After excluding those patients diagnosed with hepatotropic viral hepatitis,space occupying lesions of the liver, alcoholic liver disease and obstruction of bile duct caused by stone or malignancy and AMA/AMA-M2 positive of primary biliary cirrhosis ( PBC ), the clinical and histological characteristics were evaluated. Results Out of the 180 patients who underwent liver biopsy, 88 patients were included in the present analysis. The final diagnosis involved 15 categories of diseases, with druginduced liver injury ( DILI ) [34. 09% ( 30/88 )], autoimmune liver diseases [22.73% ( 20/88 )], and nonalcoholic fatty liver disease (NAFLD) [12. 50% ( 11/88 )] being the most common causes, following by genetic and other rare diseases. Conclusion DILI, autoimmune liver disease and NAFLD were the most common causes of abnormal liver tests in these non-viral liver diseases. Some rare diseases such as hereditary metalbolic liver disease also represent a considerable proportion in patients with abnormal liver function test.
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Objective: To study the origin, morphological structure, and functional regulation of osteoclast(OC) for further investigation on the mechanism and regulation of bone resorption. Methods: The OCs were isolated by two kinds of traditional method. Osteoclasts were isoclated from neonatal rat long bones. The cytochemistry was observed. The osteoclast-like cells (OLC) were derived from the mouse bone marrow cells in the presence of 1,25(OH)2VitD3 in vitro. Results: Both morphological and functional studies showed that the isolated cells shared some of the typical characteristics of osteoclasts, that is A. multinuclearity; B. developing spreading and pseudopodial activity when cultured on glass; C. high tartrate-resistant acid phosphatase (TRAP); D. resorption lacunae could be found when the cells were cocultured with devitalized bone slices and the number was increased as the time followed. OLC had the same histological and structural traits as the OCs by the former method. The concentration of Ca 2+ and acid phosphatase (ACP) increased gradually. Conclusion: Different kinds of method fit different experiments. The OC obtained by the first method has more activity of bone resorption. The OLC by the second method has more in quantity and can be used in the study of cell differentiation.
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<p><b>OBJECTIVE</b>To investigated the bone resorption caused by osteoclasts and modulating functions of a Chinese herb, Epimedium on bone resorption.</p><p><b>METHODS</b>Osteoclasts were separated from long-limb bones of new born rabbits, cultured with de-activated human tooth slices and glass slices, and treated with different concentrations of Epimedium. Osteoclasts on glass slices were stained by HE staining, TUNEL staining and absorption pits on tooth slices were observed by light microscope.</p><p><b>RESULTS</b>HE staining shows that the Epimedium treated cells showed shrinkage of cytosol and condensation of nuclei. Some treated cells showed breakage of nuclei. TUNEL staining shows that treated cells showed shrinkage of cytosol, dark stained nuclei and light stained cytosol. These results indicate that Epimedium can induce osteoclast to commit apoptosis. Further study shows that the number and area of absorption pits formed on treated tissues are significantly different from those on control tissues. So the effect of Epimedium on bone resorption is dose-dependent.</p><p><b>CONCLUSIONS</b>Epimedium can induce osteoclast apoptosis and inhibit bone resorption. The effect of Epimedium on bone resorption is dose-dependent.</p>