Experimental study of human bone marrow mesenchymal stem cells on regulating the biological characteristics of gastric cancer cells / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To investigate the regulatory mechanism of bone marrow mesenchymal stem cells(BMSC) on the biological profiles of KATO-III( cell lines of gastric cancer.</p><p><b>METHODS</b>Transwell cubicle was applied to build the co-cultured model in non-contact style. The differences of cell proliferation and the resistance of anti-tumour drug (5-fluoropyrimidinedione, 5-FU and Cisplatin, CDDP) between co-cultured group and single cultured group were evaluated by Cell Counting Kit 8-assay(CCK-8). The invasion ability was detected by Transwell assay. The expressions of stem cell makers, apoptosis-related factors and epithelium-mesenchymal transition (EMT)-related factors were detected by RT-PCR.</p><p><b>RESULTS</b>The proliferation ability of KATO-III( cells in co-cultured group was significantly stronger than that in single cultured group. The growth rate of KATO-III( cells in co-cultured group was significantly higher than that in single cultured group after treatment of 5-FU and CDDP(P<0.05). The mRNA expression level of Bcl-2 was significantly higher in co-cultured group KATO-III( cells(P<0.05), while the mRNA expression level of Bax was significantly lower in co-cultured group KATO-III( cells(P<0.05) in comparison with those in single cultured group. As compared to KATO-III( cells in single cultured group, the number of infiltrating-membrane cells was significantly higher (37.33±5.22 vs 14.56±2.54, P<0.01) in co-cultured group, and the mRNA expression levels of Snail and N-cadherin were significantly higher in co-cultured group KATO-III( cells (P<0.05), while the mRNA expression level of E-cadherin was significantly lower in co-cultured group KATO-III( cells (P<0.05). The expressions of CD133, Nanog and Sox-2 mRNA in co-cultured group KATO-III( cells were significantly higher than those in single cultured group(P<0.05).</p><p><b>CONCLUSIONS</b>In co-cultured model sharing non-contact style, BMSC can enhance such properties of KATO-III( gastric cancer cells as the proliferation, the invasion and the chemoresistance. Furthermore, the regulatory mechanisms may be related to the increase of the expressions of some stem cell markers in gastric cancer cells.</p>

Application status of laparoscopic gastrectomy in the treatment of gastric cancer / 国际外科学杂志

Nowadays,laparoscopy has already been widely applied to the diagnosis and treatment of kinds of diseases,improving the outcome of the patients with its less invasive advantages.In the treatment of gastric cancer,doctors always choose different methods of laparoscopic gastrectomy(LG) according to the tumor's location and its invasion and metastasis.But LG hasn't got widely application in the treatment of gastric cancer,which is meanly attributed to the exist of some Technical problems,failing to control the complications very well and the lack of evidence of long-term oncological outcomes.But with the development of new technology and the accumulation of the surgeons' experience,these problems might be conquered to a certain extent.In this review,the author will summary the application status of LG in the treatment of gastric cancer.