RESUMO
Objective To reveal the relationship between lipid distribution and age in Zhuang-Dong ethnic group in China. Methods By bioelectrical impedance analysis method of Zhuang-Dong 13 ethnic body composition in China, u inspection method, for the inspection of body composition differences between the sexes, the indicators by adopting the method of correlation analysis on body composition and age related analysis, variance analysis method was used to explore three body composition differences between age groups. Results The body fat rate was not high and did not reach the obesity level. Half of the men and more than half of the women had fat rates in the standard range. The body fat rate of Dong nationality and Bouyei nationality was higher, but that of Kelao nationality and Kelao nationality was lower. With age, there was no significant change in the upper limb fat rate of males, while the visceral fat rate and trunk fat rate increased, and the lower limb fat rate decreased. There was little change in the total fat rate and the lower limb fat rate. With age, there was no significant change in body mass index (BMI), total fat percentage, trunk fat percentage, and limb fat percentage. Women had a significantly higher percentage of body fat than men. There was no significant correlation between left and right upper limb fat rate and age in males, body fat rate, visceral fat level and trunk fat rate were significantly positively correlated with age, and left and right lower limb fat rate and age were significantly negatively correlated. The left upper limb fat rate, left and right lower limb fat rate were negatively correlated with age, and the trunk fat rate was positively correlated with age. There was no significant correlation between age and total female lipid. Conclusion The body fat of Zhuang-Dong ethnic group in China is much thinner than that of north Asian ethnic group, and it has the characteristic of sebum development level of southern Chinese ethnic group.
RESUMO
Objective:To express rationally engineered antibodies against EGFR and assess their affinity to EGFR and anti-tumor cell migration effect. Methods:L and V_H genes of humanized antibodies against EGFR were designed and synthesized. Genes encoding V_H and C_H were connected and then cloned into a pIRES based bicistronic expression vector. Gene encoding the corresponding L gene was also cloned into the same vector. 293T cells were transfected with the recombinant plasmid and the antibody expression was confirmed by Western blotting. The antibodies were purified by protein A based affinity chromatography. Binding of the humanized antibody to the EGFR was assessed by Surface Plasmon Renainance with Biacore3000, and the biological activity of the humanized antibody was determined by tumor cell invasion test.Results:Three expression vectors were constructed and the humanized anti-EGFR antibodies were expressed and purified successfully. In reducing SDS-PAGE, the antibodies exhibited two bands of approximately 25×10~3and 50×10~3, respectively. Western blot assay showed that the humanized antibodies had recognition specificity to goat-human IgG antiserum. Biacore assay revealed that the humanized antibody C3 binds to EGFR with high affinity(6.13×10~(-10)M). Cell migration test showed that C2,C3 and C5 could suppress growth and migration of tumor cells.Conclusion:Three anti-EGFR humanized antibodies (C2,C3 and C5) have been constructed and expressed successfully, and the C3 antibody retained high affinity for EGFR and showed improved inhibitory effect on tumor cell growth and migration.