RESUMO
The issues of disordering production and non-standard pesticide application are common in the production of Chinese herbal medicines. Aimed to above problems, research groups built the pollution-free and precision cultivation system of medicinal plants. This system mainly included the precise site selection of medicinal plants based on the GIS technology, modern omics-assisted breeding, metagenomics guiding the soil complex improvement, and the precise field management based on rational application of fertilizer and comprehensive control of disease. At present, the production and distribution of medicinal plants were performed in the many poor counties of the whole nation. The breeding platform of resistant varieties was built, and certificates of new and well-bred varieties were received, in the base of genetic backgrounds of the original species of medicinal plants. The disease incidences were declined after application of these resistant varieties. Additionally, chemical pesticide consumption of medicinal plants (such as Panax ginseng, P. notoginseng, Salvia miltiorrhiza, P. quinquefolium, Schisandra chinensis, Platycodon grandiflorum and P. grandiflorus etc.) reduced by 20%-80% based on the genetic testing technologies of plant diseases and insect pests and safety evaluation of pollution-free pesticides. The application of pollution-free and precision cultivation system of Chinese herbal medicines achieve significantly social, economic and ecological benefits.
RESUMO
Objective To investigate the value of T-SPOT in the diagnosis of tuberculosis (TB) in children.Methods 43 children who were admitted to a hospital and confirmed with TB between March 2015 and May 2016 were as TB group,50 children who were excluded TB as well as healthy children in the outpatient and inpatient departments of this hospital during the same period were as control group,two groups were underwent detection of TSPOT,PPD,MTB-Ab,and acid fast stain,results were compared,subgroup analysis was conducted on the positive of T-SPOT in children with different infection sites and in different age groups.Results Sensitivity and negative predictive value of T-SPOT were significantly higher than the other three methods(P＜0.05),specificity was also higher than that of PPD(P＜0.05);positive rates of T-SPOT in pulmonary tuberculosis and extrapulmonary tuberculosis were 88.9 ％ and 81.3 ％ respectively,difference was not statistically significant(P＞0.05);positive rates of T-SPOT in children ≤5 years and ＞5 years were 70.6％ and 96.2％ respectively,difference was statistically sig-nificant(P＜0.05).The sensitivity and specificity of combined detection of T-SPOT and PPD for diagnosis of TB were 95.3 ％ and 98.0％ respectively,which were both higher than the single method(P＜0.05).Conclusion The sensitivity and specificity of T-SPOT for detecting TB in children are both superior to traditional methods,T SPOT is also suitable for the detection of extrapulmonary tuberculosis,and can be used as the main method for laboratory diagnosis of tuberculosis in children,but it is uncertainty in children under 5 years of age,and should be rationally combined with PPD to improve the diagnosis of TB among children.