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Objective:To investigate the mechanism of Sishenwan in the treatment of Ulcerative Colitis and to explore the key targets and signal pathway on the treatment of Ulcerative Coltis based on network pharmacology and bioinformatics. Method:To obtain the active ingredients and predicted target genes of Sishenwan by searching the Encyclopedia of Traditional Chinese Medicine(ETCM) database, from the Gene Expression Omnibus (GEO) database and Database of Gene-disease Associations (DisGeNET), Online Mendelian Inheritance in Man (OMIM), Drug-targetDatabase (DrugBank), The Comparative Toxicogenomics Database (CTD) and Pharmacogenetics and Pharmacogenomics Knowledge Base (PharmGKB) disease database collection of Ulcerative Colitis target genes, the drug-disease protein target gene was obtained by intersecting the two and to display the results by Cytoscape software, through the algorithm of network topology to screen out the key target genes, Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out on key target genes using Gene Ontology Enrichment Analysis Softword Toolkit (GOEAST) and The Database for Annotation, Visualization and Integrated Discovery (DAVID) online tools, analyze the mechanism of Sishenwan in preventing and treating Ulcerative Colitis combined with relevant literature. Result:There are 182 active ingredients and 611 predicted targets in Sishen Wans, 914 known disease targets related to the occurrence and development of Ulcerative Colitis were retrieved through the disease database, it was concluded that the effect of Sishenwan on Ulcerative Colitis mainly involves steroid hormone-mediated signal pathway, exogenous metabolic process, positive regulation of transcription of RNA polymerase II promoter, lipid metabolic process, detoxification of cell oxidant, signal transduction and other biological functions, and participates in arachidonic acid, drug metabolism-cytochrome P450, cytochrome P450 for xenobiotics, chemical carcinogenesis and other key signal pathways. Conclusion:Sishenwan has the characteristics of multi-target, multi-channel and multi-level in the treatment of Ulcerative Colitis. Its multiple signal pathways are directly or indirectly related, participating in lipid protein metabolism, drug metabolism and anti-cancer mechanism, etc. It exerts its efficacy through comprehensive intervention on the digestive system, circulatory system, immune system and other multiple systems of the body, thus being consistent with the comprehensive effect mechanism of Ulcerative Colitis induced by multiple factors.
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Objective The aim of this study is to explore epidemic characteristics of HIV/AIDS by different route of transmission during 2011—2014 in Huzhou City,then to provide a scientific basis for the prevention and control of HIV. Methods The epidemiological case data of HIV/AIDS from 2011 to 2014 were collected in Huzhou City by China information system for disease control and prevention.Descriptive epidemiological methods were used for analysis.Results A total of 436 cases with HIV/AIDS were reported during 2011—2014 in Huzhou City,and the number of HIV/AIDS reports increased year by year.The main route of transmission include heterosexual contact,which was accounted for 56.65%.MSM transmission was accounted for 38.53%,and injection drug transmission was accounted for 3.90%.Mother to child transmission was 1 case and blood transfusion /blood products transmission were 3 cases,which accounting for 0.92%.The constituent ratio of injecting drug patients,mother to child transmission or blood transfusion /blood products patients presented declining trend (P <0.05).Patients who were infected through heterosexual contact mainly were 30 to <60 years of age (68.83%),junior middle school or below education level (80.97%),married (61.94%).Patients who were infected through MSMmainly were 15 to <45 years of age (76.19%),high school or higher education (47.62%), unmarried (51.19%),household registration of Huzhou City (52.98%).Patients who were infected through injection <br> drug mainly were 30 to <45 years of age (70.59%),primary school or lower education level (82.35%),unmarried (47.06%),household registration of other province (94.12%).Most cases were detected HIV positive through clinical tests,which accounting for 51.15%.Most HIV /AIDS of MSM,heterosexual transmission and inject -drugs were distributed in Wu Xing District,which accounting for 67.26%,51.42% and 64.71%,respectively.51.15% HIV /AIDS were detected by clinical test.Conclusion The reported cases of HIV /AIDS were still mainly transmitted by heterosexual contact in recent years in Huzhou City,and the constituent ratio of MSMtransmission has increased slightly.
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Objective To preliminarily evaluate the ability of 15 -loci variable number tandem repeats on the genotyping of clinical isolates of mycobacterium tuberculosis,and explore the suitable loci for genotyping of Mycobacterium tuberculosis in Huzhou City.Methods From 201 1 January to 201 1 April,the clinical isolates of mycobacterium tuberculosis were selected from Huzhou area to test the polymorphism of every loci by using MLVA detection method and analyze hunter-gaston discriminatory index of individual locus as well as combinations of 15 locus.Results There were two locus of Mtub-21 and Mtub-39 which had better degree of polymorphism;seven locus of ETR-A,ETR-C,MIRU-10,MIRU-23,MIRU-27,MIRU-40 and Mtub-30 had moderate degree of polymorphism;six locus of ETR-B,ETR-D,ETR-E,MIRU-16,MIRU-26 and MIRU-39 had poor degree of polymorphism.By calculation,the result showed that HGDI was 0.99 by using 15 locus to genotype,which indicated the ability to distinguish the genotype was good.Conclusion We will continue to look for other rich VNTR polymorphism locus to fit the local region fast genotyping of mycobacterium tuberculosis for higher resolution and cost effectiveness of mycobacterium tuberculosis control and prevention.