Life quality of patients after cholecystectomy / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the quality of life in patients who underwent laparoscopic and open cholecystectomy for chronic cholecystolithiasis.</p><p><b>METHODS</b>A prospective survey was made on 25 patients receiving laparoscopic cholecystectomy (LC group) and 26 patients receiving open cholecystectomy (OC group). The quality of life was measured with the gastrointestinal quality of life index (GLQI) preoperatively, and at 2, 5, 10 and 16 weeks after the operation.</p><p><b>RESULTS</b>The mean preoperative GLQI scores of all dimensions of the quality of life were 112.5 and 110.3 in the LC and OC groups respectively. In the LC group, the quality of life was not considerably reduced at 2 weeks after operation, with a mean GLQI score of 110. There was a significant improvement both in total mean score and in the aspects of symptomatology, emotional and physiological status from 5 to 16 weeks after LC operation. In the OC group, the GLQI score reduced to 102.0 at 2 weeks after surgery (P < 0.05). There were significant reductions in the aspects of symptomatology, physiological and social status as well. The GLQI scores reached to the preoperative level of 115.6 at 10 weeks after the operation (P > 0.05). The patients experienced significant improvements of GLQI at 16 weeks after OC operation (P < 0.01 or P < 0.05). The LC group showed better GLQI scores than did the OC group for up to 10 weeks postoperatively (P < 0.05).</p><p><b>CONCLUSIONS</b>LC is betler or more rapidly than OC is improving the quality of life postoperatively. The assessment of the quality of life is valuable for measuring the outcome of surgical treatment.</p>

Effects of mutant tPA gene transfer on tPA activity and adherence of endothelial cells seeded onto graft vessel / 中国普通外科杂志

Objective To evaluate the activity of tissue plasminogen activator (tPA) gene transduced endothelial cells (EC) and the cell retention on graft vessel. MethodsKG1 EC were transduced with pseudotyped vectors carrying genes coding for either the wild type tPA or mutant tPA. The supernatants were collecteJPd and assayed for tPA activity in the presence and absence of fibrin. Polytetrafluoroethylene (PTFE) vessel grafts were seeded with EC, and then exposed to an in vitro flow system for 1 h. The number of EC on grafts were counted and the retention of EC were evaluated. ResultsWT5”BZ The tPA activity of the nontransduced EC was (1 5?1 0) IU/ml, while that of wild type tPA gene transduced EC increased to (30.0?8 0) IU/ml, mutant type tPA gene transduced EC increased to (14.1?1 0) IU/ml( P

Expression of human vascular endothelial growth factor in ampullary carcinoma and its significance / 中国癌症杂志

Purpose:To investigate the expression of VEGF and its prognostic significance in ampullary carcinoma.Methods:22 resected tumor specimens from patients with ampullary carcinoma were immunohistochemically stained for VEGF and CD34 (surrogate for vessels) by streptavidin-peroxidase (S-P) method. Results:Expression of VEGF in tumor tissue was found in 50% cases of patients with ampullary carcinoma. The mean MVD for the entire group was 26.4?12.8. A significantly higher MVD(35.0?9.6) was observed in the tumors with positive VEGF expression compared with tumors with negative VEGF expression(17.7?9.3)( P