RESUMO
To study the interaction between C4b-binding protein (C4BP) and Riemerella anatipestifer (RA), we cloned duck C4BPα, conducted prokaryotic expression and prepared the polyclonal antibody by immunizing mice. Then indirect immunofluorescence assay and dot blotting hybridization assay were used to verify the interaction between C4BP and RA. The full length of duck C4BPα nucleotide sequence was 1 230 bp, with the highest similarity to chicken C4BPα (82.1%). Phylogenetic tree analysis showed that duck C4BPα and chicken C4BPα were on the same phylogenetic tree branch and the genetic evolution relationship between them was the closest. C4BPα was efficiently expressed in Escherichia coli BL21 (DE3). The recombinant proteins existed in intracellular soluble form. The titer of polyclonal antibody was more than 1:10 000 and polyclonal antibodies could specifically recognize the recombinant proteins. The results of indirect immunofluorescence assay and dot blot hybridization assay showed that RA could interact with duck C4BP. The results provide a basis to further reveal the pathogenesis of RA.
Assuntos
Animais , Camundongos , Clonagem Molecular , Proteína de Ligação ao Complemento C4b , Química , Genética , Metabolismo , Patos , Classificação , Genética , Microbiologia , Regulação da Expressão Gênica , Filogenia , Riemerella , MetabolismoRESUMO
0.05). Conclusion Fluoride contents of local wheat in some areas of Chongqing exceeded standard.
RESUMO
0.05). Compared with the control group,the OD values in the experimental groups were lower in doses of 5.0,10.0,20.0 ?g/ml,the differences were significant (P