RESUMO
Objective To explore the relationship between 1,25-dihydroxyvitamin D3(1,25-VD3)and alveolar fluid clearance(AFC)in mice in vivo,and investigate its effects in the process of lung fluid clearance. Methods KM male mice were treated with active vitamin D analogue parical-citol(daily i.p. injection)for 2 weeks,and then the in vivo AFC of these mice was measured by bovine serum albumin protein assays. western blot was applied to determine epithelial sodium channel protein levels in lungs of these mice. Results In vivo total AFC was 31.9%±3.8%in vitamin D-treated mice,and significantly lower in the vehicle-treated controls(19.7%±1.9%,P<0.05). Amiloride-sensitive AFC was increased approximate-ly 50%by vitamin D. western blot showed that the expression ofα-epithelial sodium channel was significantly elevated in paricalcitol-treated mouse lungs. Conclusion These observations suggest that vitamin D augments AFC in mice,which may be related to the augment of epithelial sodium channel protein expression. The clinical application of vitamin D therapy may ameliorate pulmonary edema of patients.
RESUMO
[Objective] To discuss the effect of intestine-dredging and Qi-benefiting method and its corresponding prescription in managing systemic inflammatory response and preventing multiple organ dysfunction syndrome (MODS). [Methods] Eighty. SD rats were randomized to control, model, low-dosage (LD) and high-dosage (HD) groups. One day before modeling, HD and LD groups were administered the preparation based on intestine-dredging and Qi-benefiting method by 60g/kg and 30 g/kg for 2 times, and control and model groups were given equal amounts of normal saline. On the 2nd day after the administration, 3 groups except the control group were modeled on the given reference. Twelve hours after modeling, the content of TNF-? and NO in serum was examined and histopathological examination of the main organs was conducted. [Results] Compared with the control group, the level of TNF-? and NO significantly increased (P