Risk factors for urinary tract infections among stroke survivors with type 2 diabetes / 中华物理医学与康复杂志

Objective:To explore the risk factors for urinary tract infection (UTI) among type 2 diabetics after an ischemic stroke.Methods:A total of 459 survivors of a first ischemic stroke who had type 2 diabetes were studied retrospectively. The 149 who had contracted a urinary tract infection (32.5%) formed the infections group; the other 310 were the no-infections group. Univariate analysis was applied to the subjects′ ages, sex, type of ischemic stroke, body mass index (BMI), history of coronary heart disease, glycosylated hemoglobin (HbAlc) levels, serum albumin (ALB) levels and serum glucose (GLU) levels. The risk factors for urinary tract infection for such patients were analyzed using binary logistic regression.Results:The univariate analysis showed that gender, age, smoking, drinking, type of ischemic stroke, coronary heart disease, as well as BMI, HbAlc and ALB levels were correlated with UTI. In the binary logistic regression analysis the significant predictors were being female, HbAlc≥6.5%, serum albumin<40g/L, obesity (BMI≥28.0kg/m 2) and the type of ischemic stroke. Conclusions:Ischemic stroke survivors with type 2 diabetes mellitus are at high risk of UTI. Being female and/or obese, a high HbAlc level, a low ALB level, and certain types of ischemic stroke are all risk factors. These findings should provide a basis for the prevention and treatment of UTIs after a stroke.

Nucleostemin specific RNAi influences cell proliferation in HeLa cells in vitro and in vivo / 中国病理生理杂志

AIM: To examine Nucleostemin (NS) expression in tumor cells, and observe the effect of NS specific RNA interference on the cell proliferation in Hela cells. METHODS: Total RNA was extracted from 6 kinds of cultured tumor lines, the NS expression level was measured by RT-PCR and Northern blot. An NS-specific siRNA expression vector was constructed to transfect HeLa cell (NS-siRNA-HeLa), and the proliferation of the cell was observed. RESULTS: NS was highly expressed in 6 kinds of tumor cells. NS expression level in the NS-siRNA-HeLa cells was remarkably reduced, and the percentage of G_0/G_1 cells increased. The neoplasm forming ability in nude mice by the NS-siRNA-HeLa cells was decreased. CONCLUSION: NS is highly expressed among tumor cells. NS-specific siRNA inhibits the entry of the cell cycle into the S phase, and remarkably reduces the proliferation ability of HeLa cells in vitro and in vivo.