RESUMO
L-carnitine [L-car] is a trimethylated amino acid required for the transformation of long chain free fatty acids into acylcarnitine and their subsequent transport into the mitochondrial matrix. It enhances the fatty acid oxidation for energy thus delaying the skeletal muscle fatigue. Objectives of this study were to determine effect of L-carnitine supplementation on skeletal muscle lactate after fatigue, to observe effect of L-carnitine supplementation on force of contraction in skeletal muscle and to estimate the time to onset of fatigue after L-carnitine supplementation. In control group, the rabbit sedated and soleus muscle was excised, Homogenized and centrifuged. Supernatant collected, was mixed with reagents of lactate test kit. Absorbance of light was read with the help of spectrophotometer and readings were noted. In experimental group, L-carnitine was given in a dose of 80mg / kg. The period of administration was two weeks. Soleus muscle was excised after giving the last dose of L-carnitine. Lactate level was measured by using lactate test kit [Randox®]. Readings for lactate measurement were noted after fatigue in both groups. Statistically significant results [p <0.05] were found in all three variables. Data was analyzed by applying [t] test in IBM SPSS Statistics - 20 software. L -carnitine delays the onset of fatigue of skeletal muscle. This was the stated alternative hypothesis
RESUMO
Calcipotriol is a vitamin D analogue that has been used now a day as monotherapy in mild to moderate psoriasis. We have conducted a placebo-controlled clinical comparative study to assess the efficacy and safety of calcipotriol in the treatment of psoriasis. To assess the efficacy and safety of calcipotriol in comparison with placebo in patients with mild to moderate psoriasis. Experimental Study. This study was conducted in the Department of Pharmacology and Therapeutics, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre [JPMC], Karachi from January 2008 to March 2008. Sixty patients with mild to moderate psoriasis were enrolled after obtaining their informed written consent and were divided into two groups, A and B. Group-A was administered calcipotriol and Group-B was considered as placebo group for three months. The study parameter [Psoriasis Area and Severity Index, PASI] score was noted after every fifteen days [02 weeks] and were evaluated statistically at the end of the study period. The significant changes in mean +/- SEM values were noted for the efficacy of calcipotriol during the period from day 0 to day 90 in group-A [calcipotriol] v/s group-B [placebo]. According to the statistical evaluation, a reduction in the symptoms of the disease was found up to 67.8% in group A, and 0.5% in group-B. The difference between the results of both groups was noted to be highly significant [P<0.001]. Calcipotriol as monotherapy is observed to be significantly superior to placebo in terms of efficacy and safety in the treatment of psoriasis