Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time

Introduction and Objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and Conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed.

Qualidade dos carboidratos de dietas consumidas por diabéticas: efeito de um programa de educação / Quality carbohydrate diets consumed by diabetics: effect of an education program

Avaliar o efeito de um Programa de Educação sobre o Índice Glicêmico (IG) e a Carga Glicêmica (CG) de dietas consumidas por mulheres diabéticas e verificar correlação destes parâmetros com medidas antropométricas e de adiposidade.

To evaluate the effect of an education program on Glycemic Index (GI) and Glycemic Load (GL) of diets consumed by diabetic women and to check correlation these parameters with anthropometricmeasures and adiposity.