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1.
Braz. j. infect. dis ; 10(3): 169-172, June 2006. ilus, tab
Artigo em Inglês | LILACS | ID: lil-435281

RESUMO

As various types of human papillomavirus (HPV) are involved in the pathogenesis of cervical cancer, correct diagnosis is of fundamental importance for screening programs. We evaluated the divergence of results between Papanicolaou cytology and hybrid capture by PCR detection of HPV DNA . A transversal study was conducted on 70 women attending private gynecological clinics in Brasilia, Brazil. PCRs were conducted with specific primers for general and high-risk HPV DNA. Based on the PCR results, hybrid capture was a superior diagnostic technique. When Papanicolaou was compared with the molecular biology methods, it was found that a positive Papanicolaou result does not necessarily indicate the presence of HPV. The agreement between PCR and hybrid capture results can be attributed to the fact that both methods detect latent infection, while Papanicolaou detects only microscopic cellular alterations.


Assuntos
Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , DNA Viral/análise , Reação em Cadeia da Polimerase , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/diagnóstico , Esfregaço Vaginal , Estudos Transversais , Papillomaviridae/genética , Infecções por Papillomavirus/patologia , Infecções por Papillomavirus/virologia , Sensibilidade e Especificidade
2.
Braz. j. infect. dis ; 6(3): 110-118, Jun. 2002.
Artigo em Inglês | LILACS | ID: lil-332325

RESUMO

The notification of sexually transmitted diseases (STD) is a prime component of well-designed public health policy. However, peculiar aspects of STD must be taken into account for the correct management of surveillance activities. Here, we describe the distribution of the most common sexually transmitted diseases among patients attended by the gynecological clinics of the principal public hospitals of Brasilia and the Federal District, Brazilian capital, during six years. A total of 142,158 patients had their cervicovaginal samples collected for Papanicolaou preparations and eventual biopsies. Diagnosis was made according to cytological and histological alterations, distinguishing among vaginal infections, and pre-cancerous and cancerous cervical lesions. We also looked at the annual prevalence of the various types of infections and alterations. There was a high prevalence of bacterial vaginosis, trichomoniasis and candidiasis, with suggestive changes over the years. Pre-cancerous and cancerous lesions increased 2.2 fold during the six years. A large proportion of the cases involved late stages of cervical cancer, indicating the necessity of prompt attendance of the population in a routine gynecological prevention program.


Assuntos
Animais , Feminino , Humanos , Infecções Sexualmente Transmissíveis/epidemiologia , Inquéritos Epidemiológicos , Brasil , Candida , Candidíase/epidemiologia , Candidíase/microbiologia , Chlamydia trachomatis , Infecções Sexualmente Transmissíveis/diagnóstico , Infecções Sexualmente Transmissíveis/patologia , Infecções Sexualmente Transmissíveis/prevenção & controle , Seguimentos , Gardnerella vaginalis , Herpes Genital , Infecções Tumorais por Vírus/epidemiologia , Programas de Rastreamento , Papillomaviridae , Prevalência , Trichomonas vaginalis , Vaginite por Trichomonas , Neoplasias do Colo do Útero , Neoplasias Uterinas , Esfregaço Vaginal , Vaginose Bacteriana
3.
Rev. Inst. Med. Trop. Säo Paulo ; 43(4): 209-212, Aug. 2001.
Artigo em Inglês | LILACS | ID: lil-298684

RESUMO

We all hope that biotechnology will answer some social and economical unavoidable requirements of the modern life. It is necessary to improve agriculture production, food abundance and health quality in a sustainable development. It is indeed a hard task to keep the progress on taking into account the rational use of genetic resources and the conservation of biodiversity. In this context, a historical perspective and prospects of the biomedical research on parasitic diseases is described in a view of three generations of investigators. This work begins with a picture of the scientific progress on biomedical research and human health over the last centuries. This black-and-white picture is painted by dissecting current advancements of molecular biology and modern genetics, which are outlined at the meaning of prospecting achievements in health science for this new millenium


Assuntos
Humanos , Animais , História do Século XIX , História do Século XX , Doenças Parasitárias , Pesquisa/história , Saúde Pública/história , Biotecnologia , Pesquisa/tendências , Saúde Pública/tendências , Técnicas Genéticas/tendências
4.
Mem. Inst. Oswaldo Cruz ; 94(suppl.1): 249-52, Sept. 1999. ilus
Artigo em Inglês | LILACS | ID: lil-245631

RESUMO

Integration of kDNA sequences within the genome of the host cell shown by PCR amplification with primers to the conserved Trypanosoma cruzi kDNA minicircle sequence was confirmed by Southern hybridization with specific probes. The cells containing the integrated kDNA sequences were then perpetuated as transfected macrophage subclonal lines. The kDNA transfected macrophages expressed membrane antigens that were recognized by antibodies in a panel of sera from ten patients with chronic Chagas disease. These antigens barely expressed in the membrane of uninfected, control macrophage clonal lines were recognized neither by factors in the control, non-chagasic subjects nor in the chagasic sera. This finding suggests the presence of an autoimmune antibody in the chagasic sera that recognizes auto-antigens in the membrane of T. cruzi kDNA transfected macrophage subclonal lines.


Assuntos
Humanos , Animais , Autoimunidade , Doença de Chagas/imunologia , DNA de Cinetoplasto , Trypanosoma cruzi , Sequência de Bases , Doença de Chagas/sangue , Genoma , Macrófagos , Trypanosoma cruzi/genética
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