RESUMO
Thirty couples suffering from habitual abortion as well as thirty normal fertile couples as a control group were subjected to HLA typing and one way mixed lymphocyte culture reaction [MLC] between females and their husbands in each group and between "females and husbands in the other groupo. There was no significant difference in the frequency, of any of the HLA-A or B antigens in females or males of recurrent abortions compared to the normal control group and another larger control group representing the general Egyptian population. However a significant increase was found in sharing of antigens as regards locus A and B and combined at both loci in habitual abortion group compared to the control group. Mothers in the abortion group were found to be significantly hyporesponsive to stimulation by their husband's lymphocytes specifically. There was no significant association between the sharing of HLA antigens and depression in MLC on individual basis, abortions. Their ages ranged from 21-35 years and the number of living children ranged from 3 to 6 children. All wives of abortion and control groups were non pregnant at the time of the study. HLA Typing: Tissue typing for HLA-A and HLA-B antigens for both partners was performed with the use of two stages lymphocyte microcytotoxicity technique [Terasaki et al, 1978] Mixed lymphocyte culture [MLC] reactivity: One way mixed lymphocyte culture was performed [Hartzman et al, 1971] and the following responses were studied: 1.Cellular response of cells from the wives: each stimulated, by her husband in both study and control groups 2.Cellular response of the wives in the abortion group stimulated by men from the control group 3 Cellular response of the wives in the control group stimulated by men from the study group N.Bs:Every MLC combination was done in triplicate. Results of MLC were expressed as stimulation index [SI] and relative response [RR] as follows: SI = Test mean count per minute [cpm] Autologous mean [cpm] Where: Test cpm = Mean counts per minute for a woman's cells [female responder] against mitomycin treated husband's stimulator cells [male stimulator]
Assuntos
Humanos , Masculino , Feminino , Antígenos HLA-A/sangue , Antígenos HLA-B/sangue , Histocompatibilidade , Teste de Cultura Mista de LinfócitosRESUMO
This study was carried out on 30 collections of houseflies from a locality representing the rural community, in a period covering the four seasons of the year. Flies were examined for the presence of different bacterial species and for C. trachomatis on their external surface and in internal contents. The percentage of bacterial culture positivity for external parts and internal contents was 73% and 90%, respectively. However, isolation of C. trachomatis on McCoy cells was not successful, but chlamydial antigen was detected in 14 specimens out of 60 [external and internal parts of houseflies]
Assuntos
Chlamydia trachomatis , Infecções Oculares BacterianasRESUMO
A significant increase of the serum immunoglobulins [IgG, M and A] in patients with schistosomal hepatic fibrosis with and without ascites was noticed as compared with serum of normal persons. As regards the concentrations of immunoglobulins in the ascitic fluid, a high percentage of these immunoglobulins was observed. To define the role of humorally mediated antimicrobial activity of human ascitic fluid, bactericidal assays, in addition to the estimation of immunoglobulins, was performed on cell-free ascitic fluid from 10 patients with bilharzial hepatic fibrosis and ascitis. Assays using E.coli and K.pneumoniae showed bactericidal or bacteniostatic activity for both organisms in 8 out of 10 fluids. While the other 2 specimens of fluids showed no inhibitory activity against them. The same results were obtained using Staph. aureus. However no inhibitory activity was seen for Strept. faecalis. The significant increase in IgG, M and A in both serum and ascitic fluid of schistosomal hepatic fibrosis patients with ascitis, together with the inhibitory activity against gram negative organisms of the ascitic fluid may account for the absence of peritonitis in these patients inspite of repeated tapping and the absence of inflammatory reactions around the site of tapping