RESUMO
Background: the present study was conducted to evaluate and compare a fast-ELISA [F-ELISA] method versus standard- ELISA [S-ELISA] to diagnose human fasciolosis
Methods: serum samples were obtained from 35 individuals infected with fasciolosis, 27 infected with other parasitoses and 22 from healthy people. The samples were examined with S-ELISA [30-minute incubation periods] and F-ELISA [10-minute incubation periods] for total antibody response against fasciolosis
Results: the optimum conditions for S-ELISA and F-ELISA were respectively as follows: antigen 10 and 5 micro g/mL, sera 1:500 dilution for both, peroxidase-conjugated goat anti-human IgG diluted 1:7000 and 1:10000. Data were analyzed using SPSS version 11.0. Cut-off vale for S-ELISA and F-ELISA was determined as 0.56 and 0.42, respectively. The sensitivity, specificity; positive, and negative predictive values were detected as 97.2%, 100%, 94.6%, and 95.6% for both tests
Conclusion: cut-off values, sensitivity, specificity, and other important parameters of the two evaluated tests determined that the F-ELISA method could be used with no detectable difference