Cryopreservation of Cartilage for Transplantation in Rhinoplasty: A Literature Review / 대한이비인후과학회지

Although many rhinosurgeons still use cryopreserved septal or rib cartilage for grafts in rhinoplasty, there is no consensus whether it is an effective method preserving the chondrocyte viability. To propose a best option in the ex-vivo preservation of cartilage by literature review. A critical review of the published literature using PubMed, EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL) with particular emphasis on studies which include cartilage cryopreservation was performed. Using databases, articles published from 1956 through 2011 for all available studies reporting cryopreservation of cartilage were investigated. Keywords used in this search were "cryopreservation", "nasal cartilage", "articular cartilage", "chondrocytes", and "rhinoplasty". We could confirm that isolated chondrocytes can be effectively cryopreserved, yet the survival of chondrocytes in cryopreserved intact cartilage tissue is inadequate and extremely variable. Hypothermic preservation of intact cartilage in culture media showed good cell viability and function up to 2 weeks only. Though it is possible to cryopreserve one cell-layer tissue with the preservation of a high percentage of the initial cells, successful methods for cryopreservation of whole cartilage have not been achieved despite numerous efforts world-wide. Preserving the whole cartilage tissue at 4℃ in culture media proved that the tissue showed good viability with metabolic activity for 2 weeks.

Base of tongue tuberculosis: a case report

We report a rare case of base of tongue tuberculosis following pulmonary tuberculosis. Patient presented to us with chief complaints of sore throat and pain on swallowing for period of 3 months. On examination with 70 degree telescope, we observed an ulcer on right side of base of tongue. The edges of the ulcer appeared to be undermined with whitish slough at the centre of the ulcer. Examination of neck showed a multiple small palpable middle deep cervical lymph nodes on right side of neck. Biopsy of the ulcer was taken, which showed granulomatous inflammation, suggestive of tuberculosis. Laboratory investigations revealed a raise in erythrocyte sedimentation rate, sputum for acid fast bacilli was strongly positive. Chest X ray was performed for patient showed multiple areas of consolidation. Patient was referred to chest clinic for further management of tuberculosis and was started on anti-tuberculous drugs. In conclusion tuberculosis of oral cavity is rare, but should be considered among one of the differential diagnosis of the oral lesions and biopsy is necessary to confirm the diagnosis