RESUMO
Insulin like growth factor-1 has incriminated as a key role in the pathogenesis of immediate and late diabetic complications. IGF-1 concentration changes either in blood or renal tissues may contribute to the pathogenesis of diabetic nephropathy. The objective of this study is to compare the level of urinary and serum IGF-1 in diabetics and non-diabetic subjects and to predict the relation between IGF-1 and development of diabetic nephropathy. It is released from various organs with the liver being the most important source. The present study assessed urinary and serum 1GF-1 in 24 patients, twelve of them are diabetics with microalbuminuria and twelve of them are diabetic with overt nephropathy and also in twelve control non-diabetic subjects of matched age and sex. Every group contain six males and six females, the differentiation between diabetic groups was based on Cambur-lll and Micral-l tests. The immunoassay of IGF-1 is a "sandwich type assay" by using mouse monoclonal antibodies directed against two different epitopes of IGF-1 which are employed in the kit. Serum IGF-1 showed statistically non-significant difference between the three test groups. Urinary IGF-1 showed marked significant difference between the three test groups. The highest value was in overt nephropa thy group [group III] [0.800:0.100 pg/ ml], then microalbuminuria diabetic group [group II] [0.408:0.007 pg/ml]. The lowest value was found in control group [group I] [0.189+0.0028 pg/ml], p value <0.01 when comparing group III with group I. Also urinary IGF-1 showed a positive correlation with the diabetic duration, mean blood pressure and serum creatinine level [P<0.001]. These results indicate that urinary 1GF-1 may reflect the important role of tissue IGF-1 in the pathogenesis and development of diabetic kidney disease