Effect of r-interferon-gamma on inerleukin-1 production by keratinocytes / 대한피부과학회지

It now appears that in addition to being a target for immunologic injury, the epidermis actively participate in various immunologic processes and thus may serve as the peripheral limb of the immune system. For example, keratinocytes produce the cytokines including IL-1 and in certain inflammatory diseases characterized by accumulation of T lymphocytes, kerationchtes aberrantly express HLA-DR antigen. In this study, the effect of interferon-gamma on keratinocytes IL-1alpha production were invesigated by radioimmunoassay, using as a model cultured keratinocytes stimulated with 30U/ml of recombinant-Interferon-gamma(IFN), IFN and 1microgram/ml of PMA(IFN-PMA), IFN-gamma and 25microgram/ml of LPS(IFN-LPS), PMA, and LPS. The expression of HLA-DR antigen and the effect of IFN-gammaon the proliferation of kerationcytes were also evaluated. The results were as follows: 1. The proliferation of keratinocytes was significantly inhibited in the IFN, IFN-PMA, and IFN-LPS groups compared with the control. The proliferation of kerationcytes in PMA and LPS groups was not inhibited. 2. The percent of HLA-DR positive kerationcytes was 62.5+/-3.1%, and 65.8+/-2.6% in IFN, IFN-PMA, and IFN-LPS group, respectively. There were no HLA-DR expression in the control, PMA, and LPS groups. 3. The amounts of IL-lalpha were 22.58+/-8.41, 49.32+/-13.01, 57.02+/-14.99, 96.98+/-43.17, 22.30+/-4.26, and 44.60+/-20.51 fmol in supernatant of control, IFN, IFN-PMA, IFN-LPS, PMA, and LPS group, respectively. The differences between IFN and control, IFN-PMA and PMA, and IFN-LPS and LPS were significant (P<0.05). The produciton of IL-1alphawas enhanced by IFN, but not PMA and LPS. The IL-1alpha induced by IFN and PMA was higher than that induced by IFN or PMA alone. The IL-1alpha induced by IFN and LPS was higher tthan that induced by IFN or LPS alone. 4. The amounts of IL-1alpha were 59.82+/-11.57, 70.15+/-25.22, 73.50+/-17.15, 63.67+/-32.38, 48.62+/-4.81, and 50.92+/-15.01 fmol in cell lysate of control, IFN, IFN-PMA, IFN-LPS, PMA, PMA, and LPS groups, respectively. The amount of IL-1alpha in IFN-PMA was significantly increased as compared to PMA(P<0.05). 5. The total amounts of IL-1alpha were 82.40+/-8.98, 119.47+/-21.88, 130.52+/-8.12, 160.66+/-34.51, 70.92+/-1.15, and 95.53+/-27.89 fmol in the control, IFN, IFN-PMA, IFN-LPS, Pma, and LPS groups, respectively, The differences between IFN and control, control, IFN-PMA and PMA and IFN-LPS and LPS were signigicant (P<0.05) the production of IL-1alpha was enhanced by IFN, but not PMA and LPS. The IL-1alpha induced by IFN and PMA was higher than that induced by PMA. The IL-1alpha induced by IFN and LPS was higher than that induced by IFN or LPS alone. In summary, the results indicate that when kerationcytes are activated by IFN-gamma, they are actively participating in the immunologic reaction by the increasing production of IL-1alpha.

A Case of Childhood Dermatomyositis / 대한피부과학회지

We report a case of childhood dermatomyositis t.hat developed in a 10-year-old boy. Skin lesion showed malar erythema, Gottron's papule, and poikiloderma those were compatible with dermatomyositis. And other clinical, laboratory, and histopathologic findings supported the diagnosis of derrnatomyositis. We treated him with oral corticosteroid, the lesion was improved and regular periodic follow up was attempted. From summary of all data of patient, this case was typical Brunsting type of childhood dermatomyositis.

Effect of Acupuncture on Histamine Induced Itch / 대한피부과학회지

We cairried out this study to find the possibility of itch control with acupuncture under the hypothesis that antipruritic effect also exists if the itch sensation is closely allied to pain. We evaluated the effect of acupuncture on histarnine induced itch, flare and wheal in 20 healthy male volunteers aged 24 32 years old and compared it with the effect of pseudoacupuncture on them and that of no intervention on them. The results were as follows: 1. Mans of itch intensity and duration were decreased in A(session with acupuncture) and P(session with Pseudoacupuncture), compared to those of N(session with no intervention), and A was more effective in decreasing the itch intensity and its duration than P. 2. Means of maximal sizes of flare and wheal were smaller in A and P, but there was no remarkable difference between A and P.

Two Cases of Pseudocyst of Auricle / 대한피부과학회지

We present two cases of pseudocyst of auricle which occured in 59-year-old man and 21-year-old man. Each showed bean sized non-inflammatory cystic swelling of upper part of right auricle with slight tenderness and the cystic content was serosanguinous fluid. Cystic wall was composed of fibrovascular tissue replacing degenerated cartilage without lining epithelium.

A Case of Xeroderma Pigmentosum / 대한피부과학회지

The role of the immune system in pathogenesis of skin cancer has been attracted increasing attention in recent years. Some limited studies and observations suggest that altered immune status may be a factor in the initiation and growth of cutaneous malignancies. Authors experienced a typical case of xeroderma pigmentosum in a 6 year-old girl, who has been suffered from hyper and hypopigmented macules on sun-exposed area, photophobia and also noted eroded lesion on the lower lip. The biopsy finding of the hyperpigmented macule showed that of freckle and the finding of actinic cheilitis was observed from the specimen of eroded 1esion on the lower lip. We performed immunologic test and obtained some evidences of immunologic defect; such as decreased numbers of total T cells, T-suppressor cell and B cell in our patient.

A Case of Epidermolysis Bullosa Dystrophica / 대한피부과학회지

Epidermolysis bullosa dystrophica(EBD) is a rare, hereditary and chronic mechanobullous disease characterized by blistering and erosions of the skin in response to even minor trauma. A 5-year old girl visited with a complaint of continuous vesicobullous skin eruptions since birth. On examination, bullae, vesicles, atrophic scars, crusts and ero:ions were scattered on whole body. Loss of finger and toe nails, partial syndactyly of all toe webs and milia on dorsa of feet were noticed. We present a case of EBD that seems to be a recessive type considering the absence of family history with histopathologic and electron microscopic findings.