RESUMO
OBJECTIVE: To explore the protective effect of 2, 3, 5, 4'-tetrahydroxystibene-2-O-β-D-glucoside(TSG) on 1-methy-4-phenylpyridinium(MPP+) induced apoptosis in PC12 cells and its possible mechanism. METHODS: The cell viability was measured by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide(MTT).The morphological change of PC12 cells was observed by Hoechst 33258 staining. The intracellular ROS/NO level was examined by using DCFH-DA/DAF-FM DA. The expression level of Nrf2, Keap1, SOD1, SOD2, CAT protein was detected by Western blotting. RESULTS: Compared with the control group, the ratio of cell survival decreased to (51.3±2.2)% (P<0.01) after MPP+ treatment with PC12 cells for 24 h.Cell viability was increased to(60.1±1.5)%, (74.2±2.1)%, (82.1±1.5)% (P<0.05) respectively after exposure with 10, 50 μmol·L-1 TSG. Cytochromatin concentration reduced and show the relation between quantity and result. Furthermore, TSG inhibited the MPP+-induced increase reactive oxygen species/nitric oxide (ROS/NO) in PC12 cell, and counteracted the over expression of Keap1 and low-expression of Nrf2, SOD1, SOD2, CAT. CONCLUSION: TSG plays a possible neuroprotective role in MPP+ induced apoptosis of PC12 cells by a concentration-dependent manner, and the mechanism of the effects of TSG may be involved in facilitating Nrf2/keap1 activation.