Mitochondria associated endoplasmic reticulum membranes in cisplatin induced ovarian cancer cell apoptosis / 中国病理生理杂志

AIM: To explore the structural change of mitochondria associated endoplasmic reticulum mem -branes(MAMs)in SKOV3 cells exposed to cisplatin.METHODS:The SKOV3 cells were treated with cisplatin at con-centration of 6 mg/L.The protein levels of active caspase-3,as well as the colocalization of B-cell receptor-associated pro-tein 31(BAP31)and voltage-dependent anion channel protein 1(VDAC1)in the SKOV3 cells were determined by the method of indirect immunofluorescence.The apoptotic rate of the SKOV3 cells was analyzed by flow cytometry.The struc-tural change of MAMs was observed under transmission electron microscope.RESULTS:Under the confocal microscope, we found that cisplatin increased the protein levels of active caspase-3 as well as colocalization of BAP31 and VDAC1 in the SKOV3 cells.The results of flow cytometry demonstrated that cisplatin increased the apoptotic rate of the SKOV 3 cells (P<0.05).The results of transmission electron microscopy showed that cisplatin induced increase in mitochondrial -asso-ciated membrane structures(P<0.05).CONCLUSION:Cisplatin induces SKOV3 cell apoptosis with increased MAMs contacts.MAMs may play a role in cisplatin induced SKOV3 cell apoptosis.

Analysis of treatment and prognosis in 34 patients with anorectal malignant melanoma / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To explore the prognostic factors of anorectal malignant melanoma (ARMM).</p><p><b>METHODS</b>Medical records and follow-up data of 34 patients with ARMM treated in the Chinese PLA General Hospital from March 1993 to November 2011 were analyzed retrospectively.</p><p><b>RESULTS</b>There were 26 abdominoperineal resections(APR) and 8 wide local excisions (WLE). Twenty patients underwent postoperative adjuvant therapy, including chemotherapy in 14 cases, radiotherapy in 2 cases, traditional Chinese medicine therapy in 4 cases and immunotherapy in 16 cases. Postoperative follow-up was carried out in all the patients and the mean follow-up period was 27 months. The 1-, 3- and 5-year overall survival rates were 76.3%, 39.6% and 20.6% respectively, while the 1-, 3- and 5-year disease-free survival rates were 60.6%, 30.8% and 12.8% respectively. APR and postoperative immunotherapy could significantly reduce the local recurrence rate. According to the Kaplan-Meier method, gross type of tumor, mural involvement, lymph metastasis, and clinical staging had significant effects on overall survival, while lymph metastasis and postoperative immunotherapy on disease-free survival. Cox proportional hazards model indicated that the clinical staging and postoperative immunotherapy were significant predictive factors.</p><p><b>CONCLUSIONS</b>Early diagnosis and correct choice of surgical method are the keys to the treatment. Postoperative immunotherapy can prolong disease-free survival.</p>

Robotic anterior resection of rectal cancer: technique and early outcome / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The Da Vinci system is a newly developed device for colorectal surgery. With advanced stereoscopic vision, lack of tremor, and the ability to rotate the instruments surgeons find that robotic systems are ideal laparoscopic tools. Since conventional laparoscopic total mesorectal excision is a challenging procedure, we have sought to assess the utility of the Da Vinci robotic system in anterior resections for rectal cancer.</p><p><b>METHODS</b>Between November 2010 and December 2011, a total of 22 patients affected by rectal cancer were operated on with robotic technique, using the Da Vinci robot. Data regarding the outcome and pathology reports were prospectively collected in a dedicated database.</p><p><b>RESULTS</b>There were no conversions to open surgery and no postoperative mortality of any patient. Mean operative time was (220 ± 46) minutes (range, 152 - 286 minutes). The median number of lymph nodes harvested was (14.6 ± 6.5) (range, 8 - 32), and the circumferential margin was negative in all cases. The distal margin was (2.6 ± 1.2) cm (range, 1.0 - 5.5 cm). The mean length of hospital stay was (7.8 ± 2.6) days (range, 7.0 - 13.0 days). Macroscopic grading of the specimen was complete in 19 cases and nearly complete in three patients.</p><p><b>CONCLUSIONS</b>Robotic anterior resection for rectal surgery is safe and feasible in experienced hands. Outcome and pathology findings are comparable with those observed in open and laparoscopy procedures. This technique may facilitate minimally invasive radical rectal surgery.</p>

Laparoscopy-assisted D2 radical distal gastrectomy for advanced gastric cancer: initial experience / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Laparoscopy-assisted radical gastrectomy is gaining acceptance for treating early gastric cancer. However, few reports concerning the effectiveness of laparoscopy-assisted D2 radical distal gastrectomy (LADG) for advanced gastric cancer or data comparing the results obtained after open distal gastrectomy (ODG) are yet available. The aim of this study was to evaluate the method, feasibility and clinical result of LADG for advanced gastric cancer.</p><p><b>METHODS</b>A retrospective study was performed comparing LADG and ODG for advanced gastric cancer. Seventy-eight patients who underwent LADG were compared with 90 patients who underwent ODG in terms of pathologic findings, operative outcome, and complications.</p><p><b>RESULTS</b>There was no conversion to open surgery in the LADG group and no postoperative mortality of any patients. There were no significant differences between LADG and ODG in operative time ((245 +/- 35) vs (220 +/- 20) minutes), complication rate (7.7% vs 10.0%), and number of lymph nodes (23.5 +/- 6.0 vs 21.0 +/- 7.5), while the blood loss was less after LADG ((110 +/- 25) vs (196 +/- 30) ml, P < 0.05). The time to postoperative flatus and postoperative hospital stay were shorter after LADG ((73.0 +/- 8.5) vs (102.0 +/- 10.5) hours, and (8.6 +/- 1.2) vs (12.1 +/- 2.5) days, P < 0.05, respectively).</p><p><b>CONCLUSION</b>LADG for advanced gastric cancer is feasible, safe, and minimally invasive.</p>