An experimental study on recombinant adenovirus p53 transfected in oral dysplastic epithelial cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate and evaluate the appropriate virus titer and transfection efficiency of recombinant adenovirus p53 into the oral dysplastic epithelial cells (POE-9n) and provide reference for oral precancerosis research.</p><p><b>METHODS</b>The transfection sensitivity of adenovirus into oral dysplastic epithelial cells was evaluated by the recombinant adenovirus p53 containing green fluorescent protein (rAd-GFP). Different titre rAd -p53 was transfected into oral dysplastic epithelial cells to evaluate the effects of rAd-p53 on cell proliferation inhibition by MIT assay. The expression of exogenous p53 gene in POE-9n cells was detected by immunocytochemistry.</p><p><b>RESULTS</b>More than 95% POE-9n cells were transfected by rAd-GFP with MOI from 100 to 500 and there was no statistical difference between different MOI values (r=-0.124, P>0.05). It was found that rAd-p53 had significant inhibition effects on POE-9n cell proliferation with MOI from 100 to 500, and there were no significant differences at 96 h and 120 h after the transfection on cell proliferation inhibition (P>0.05). P53 protein was well expressed in rAd-p53 transfected POE-9n cells.</p><p><b>CONCLUSION</b>Exogenous p53 can be successfully transfected into POE-9n cells by rAd-p53 and the virus titer of MOI 100 was high enough to ensure efficient transfection.</p>

Altered gene expression of lymphatic endothelial cells in oral cancer / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To explore altered molecular phenotype of lymphatic endothelial cells (LEC) induced by oral cancer cells. It is relevant to develop therapeutic strategies for blocking oral cancer spread through lymphatic vessels.</p><p><b>METHODS</b>LEC were co-cultured with oral cancer cells in a model. Differential gene expression profiles between tumor-derived lymphatic endothelial cell (TLEC) and LEC were analyzed by Human Genome U133 Plus 2.0 GeneChip arrays. Differential expression genes of functional similarity were classified.</p><p><b>RESULTS</b>Differential gene expression profiles revealed that 677 unique genes had at least a twofold change in expression level between the two groups, 384 were overexpressed, and 293 were underexpressed in TLEC. These genes were related to cell adhesion, apoptosis, cell motility, development, and angiogenesis. In addition, some genes were involved in signal transduction, immune response, cell metabolism, and so on.</p><p><b>CONCLUSION</b>LEC and TLEC are distinct at the molecular level. A novel therapeutic strategy of lymphatic metastasis is encouraging and anticipated based upon manipulation of LEC responses to oral cancer cells.</p>

The study of apoptosis induction effect of vitamin E succinate on Tca8113 human tongue cancer cells / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the apoptosis induction effect of vitamin E succinate (VES) on Tca8113 cells and its possible mechanisms.</p><p><b>METHODS</b>The proliferative activity of Tca8113 was assessed by methyl thiazolyl tetrazolium (MTT) assay. After Tca8113 cells were treated with different concentrations of VES, apoptotic rates were analyzed by flow cytometry (FCM). Fas monoclonal antibody was used for the blocking test. Fas expression was detected by immuocytochemistry(SABC assay) and FCM.</p><p><b>RESULTS</b>VES demonstrated a significant growth inhibitory effect and apoptosis induced effect on the Tca8113 cells in a dose- and time-dependent manner. Fas neutralizing antibody can block the apoptosis induced by VES. After the administration of VES, the expression of Fas protein increased and the kytoplasm staining enhanced. Proteinum quantitative analysis showed that the mean fluorescence intensity increased.</p><p><b>CONCLUSION</b>VES can induce apoptosis in human tongue cancer cells, and the up-regulation of the cell surface Fas protein may play an important role in the process.</p>

Biologic analysis of recombinant human adenovirus-p53 injection in patients with oral leukoplakia / 华西口腔医学杂志

<p><b>OBJECTIVE</b>Advances in tumor biology and clinical trials indicate that p53 transfer is an alternative therapy for head and neck squamous cell carcinoma. The aim of this study is to evaluate the biologic activity of multiple intraepithelial injections of Ad-p53 in patients with dysplastic oral leukoplakia, which is the most common premalignant lesion of the oral squamous cell carcinoma.</p><p><b>METHODS</b>From 2006 to 2007, 18 Chinese patients clinically and histopathologically diagnosed as dysplastic oral leukoplakia were recruited for this study. On a 15-day cycle, intraepithelial injections of Ad-p53 were administered once every three days at dose levels dependent upon lesion size/dose escalation sequence (1 x 10(8) vp). 24-48 h after the last injection, incisional biopsy were performed, and immunohistochemistry was used to examine the protein expression of P53 and P21(CIP/WAF).</p><p><b>RESULTS</b>In the postreatment patients, P53 protein and P21(CIP/WAF) protein expression were significantly enhanced (100%, 89.9%, respectively) and statistical analysis revealed the expression of P53 protein had a positive correlation with that of P21(CIP/WAF) protein (r=0.598, P<0.01).</p><p><b>CONCLUSION</b>Intraepithelial injections of gendicine is biologically active in patients with dysplastic oral leukoplakia. It may be a promising treatment for oral leukoplakia.</p>

Relationship between the expression of cyclooxygenase-2 and microvessel density in oral squamnous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To detective the relationship between cyclooxygenase-2 (COX-2) and angiogenesis in oral squamous cell carcinoma (OSCC) and its clinical significance through observing the expression of COX-2 and determining microvessel density (MVD) in OSCC.</p><p><b>METHODS</b>PV-9000 immunohistochemistry was used to determine the expression of COX-2 and CD34, which was used to determine MVD, in 76 OSCC tissues and 12 normal oral mucosa tissues.</p><p><b>RESULTS</b>Overexpression of COX-2 was detected in OSCC, and was more intense compared with normal epithelium (P < 0.001). The high expression of COX-2 in OSCC was related to neck lymphnode metastasis, tumor size, TNM stage and histological grade (P <0.05). The MVD value in COX-2-positive group was much higher than that in COX-2-negative group (P < 0.01) and that in normal oral mucosa tissues (P < 0.01).</p><p><b>CONCLUSION</b>The high expression of COX-2 in OSCC was significantly associated with MVD, neck lymphnode metastasis, tumor size, TNM stage and histological grade. COX-2 might be one of the important factors in the angiogenesis of OSCC.</p>