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1.
Artigo em Chinês | WPRIM | ID: wpr-1021899

RESUMO

BACKGROUND:Tissue engineering has brought new hope to the clinical challenge of liver failure,and the preparation of plant-derived decellularized fiber scaffolds holds significant importance in liver tissue engineering. OBJECTIVE:To prepare apple tissue decellularized scaffold material by using fresh apple slices and a solution of sodium dodecyl sulfate,and assess its biocompatibility. METHODS:Fresh apples were subjected to decellularization using phosphate buffer saline and sodium dodecyl sulfate solution,separately.Afterwards,the decellularized apple tissues and apple decellularized scaffold materials were decontaminated with phosphate buffer saline.Subsequently,scanning electron microscopy was used to assess the effectiveness of decellularization of the apple materials.Adipose-derived mesenchymal stem cells were extracted from the inguinal fat BALB/C of mice,and their expression of stem cell-related markers(CD45,CD34,CD73,CD90,and CD105)was identified through flow cytometry.The cells were then divided into a scaffold-free control group and a scaffold group.Equal amounts of adipose-derived mesenchymal stem cells were seeded onto both groups.The biocompatibility of the decellularized scaffold with adipose-derived mesenchymal stem cells was evaluated using CCK-8 assay,hematoxylin-eosin staining,and phalloidine staining.Cell adhesion and growth on the scaffold were observed under light microscopy and scanning electron microscopy.Furthermore,the scaffold was subdivided into the non-induced group and the hepatogenic-induced group.Adipose-derived mesenchymal stem cells were cultured on the decellularized apple scaffold,and they were cultured for 14 days in regular culture medium or hepatogenic induction medium for comparison.Immunofluorescent staining using liver cell markers,including albumin,cytokeratin 18,and CYP1A1,was performed.Enzyme-linked immunosorbent assay was used to detect the secretion of alpha fetoprotein and albumin.Additionally,scanning electron microscopy was employed to observe the morphology of the induced cells on the scaffold,verifying the expression of liver cell-related genes on the decellularized scaffold material.Finally,the cobalt-60 irradiated and sterilized decellularized apple scaffolds were transplanted onto the surface of mouse liver and the degradation of the scaffold was observed by gross observation and hematoxylin-eosin staining after 28 days. RESULTS AND CONCLUSION:(1)The scanning electron microscopy results revealed that the decellularized apple scaffold material retained a porous structure of approximately 100 μm in size,with no residual cells observed.(2)Through flow cytometry analysis,the cultured cells were identified as adipose-derived mesenchymal stem cells.(3)CCK-8 assay results demonstrated that the prepared decellularized apple tissue scaffold material exhibited no cytotoxicity.Hematoxylin-eosin staining and phalloidine staining showed that adipose-derived mesenchymal stem cells were capable of adhering and proliferating on the decellularized apple tissue scaffold.(4)The results obtained from immunofluorescence staining and enzyme-linked immunosorbent assay revealed that adipose-derived mesenchymal stem cells cultured on the decellularized apple scaffolds exhibited elevated expression of liver-specific proteins,including albumin,alpha-fetoprotein,cytokeratin 18,and CYP1A1.These results suggested that they were induced differentiation into hepatocyte-like cells possessing functional characteristics of liver cells.(5)The decellularized apple scaffold implanted at 7 days has integrated with the liver,with partial degradation of the scaffold observed.By 28 days,the decellularized apple scaffold has completely degraded and has been replaced by newly-formed tissue.(6)The results indicate that the decellularized scaffold material derived from apple tissue demonstrates favorable biocompatibility,promoting the proliferation,adhesion,and hepatic differentiation of adipose-derived mesenchymal stem cells.

2.
Acta Pharmaceutica Sinica B ; (6): 4127-4148, 2023.
Artigo em Inglês | WPRIM | ID: wpr-1011165

RESUMO

Articular cartilage (AC) injuries often lead to cartilage degeneration and may ultimately result in osteoarthritis (OA) due to the limited self-repair ability. To date, numerous intra-articular delivery systems carrying various therapeutic agents have been developed to improve therapeutic localization and retention, optimize controlled drug release profiles and target different pathological processes. Due to the complex and multifactorial characteristics of cartilage injury pathology and heterogeneity of the cartilage structure deposited within a dense matrix, delivery systems loaded with a single therapeutic agent are hindered from reaching multiple targets in a spatiotemporal matched manner and thus fail to mimic the natural processes of biosynthesis, compromising the goal of full cartilage regeneration. Emerging evidence highlights the importance of sequential delivery strategies targeting multiple pathological processes. In this review, we first summarize the current status and progress achieved in single-drug delivery strategies for the treatment of AC diseases. Subsequently, we focus mainly on advances in multiple drug delivery applications, including sequential release formulations targeting various pathological processes, synergistic targeting of the same pathological process, the spatial distribution in multiple tissues, and heterogeneous regeneration. We hope that this review will inspire the rational design of intra-articular drug delivery systems (DDSs) in the future.

3.
Chinese Journal of Surgery ; (12): 894-897, 2017.
Artigo em Chinês | WPRIM | ID: wpr-809638

RESUMO

AJCC Esophageal Cancer Staging System, 8th edition will be implemented on January 1, 2018. The N staging in 8th edition of staging system remains following 7th edition based on the number of metastatic nodes, except the limited revision of the regional lymph node map. N staging revision was reviewed from the simple definition of negative (N0) and positive (N1) lymph node(s) to the positive node number based proposal (7th edition). The 7th edition staging system, especially the N staging, were proved with more advantages on distinguishing disease progression and predicting prognosis of the esophageal cancer. On other hand, the disadvantages of 7th edition N staging are discussed. The refined N staging based on the number of metastatic node station is introduced. The extent and station of metastatic node could better reflect the disease progression and prognosis according to our research. The controversy on N staging of esophagogastric junction cancer is discussed as well. Other reported N staging associated index including lymph node ratio, lymphatic vessel invasion and biomarkers are reviewed and evaluated.

4.
Chongqing Medicine ; (36): 939-940,943, 2015.
Artigo em Chinês | WPRIM | ID: wpr-600423

RESUMO

Objective To investigate the status of dental caries of deciduous teeth among preschool children from private kin-dergarten in Chongqing city.Methods According to the criteria recommended by The Third National Oral Health Investigation,the deciduous caries of 2 713 preschool children aged 3 to 6 years from 40 private kindergartens in Yuzhong district were examined.The prevalence of dental caries and the mean dmft were calculated.The results were analyzed using SPSS21.0 software package. Results The prevalence of dental caries was 61.04%.The mean dmft score was 3.12,the filling rate was 0.4%.The caries preva-lence of 3-,4-,5-,6-year-old children were found to be 42.51%,59.53%,67.76% and 74.85%,and the mean dmft score were 2. 71,3.68,3.96 and 4.09.The prevalence of dental caries and mean dmft increased gradually as the children was statistically signifi-cant(P 0.05).Conclusion Deciduous dental caries of preschool children from private kindergartens was an important problem in Chongqing city,and the filling rate was very low.

5.
China Modern Doctor ; (36): 16-18, 2014.
Artigo em Chinês | WPRIM | ID: wpr-1037064

RESUMO

Objective To observe the efficacy of mannatide injection and nedaplatin injected in thoracic cavity in the treatment of malignant pleural effusion. Methods Ninety-four patients with malignant pleural effusion were randomly divided into two groups. The observation group of 48 patients were given mannatide injection and nedaplatin,and con-trol group of 46 patients were given only nedaplatin. Effectiveness and adverse reactions were observed after treatment. Results The effective rate was 85.4% in observation group, hihger than that in control group (69.6%)(P<0.05); The incidence rates of gastrointestinal tract and bone marrow and chest pain of observation group were 35.4%,22.9% and 18.8%. Compared with control group, the incidence of observation group had significantly lowered. There were signifi-cant differences between the two groups(P<0.05). Conclusion Mannatide injection combined with nedaplatin is more efficient and less adverse reaction for malignant pleural effusion, so it is worth popularizing.

6.
Artigo em Chinês | WPRIM | ID: wpr-231823

RESUMO

<p><b>OBJECTIVE</b>To investigate the status of deciduous dental caries among preschool children in Chongqing city and to compare the oral health status of preschoolers from public and private kindergarten.</p><p><b>METHODS</b>According to the criteria recommended by The Third National Oral Health Investigation, the deciduous caries of 5 030 preschool children aged 3 to 6 years old from 40 private kindergartens and 16 public kindergartens in Yuzhong district of Chongqing were examined. The prevalence of dental caries and the mean dmft and filling rate were calculated. The results were analyzed using SPSS 21.0 software package.</p><p><b>RESULTS</b>Maxillary deciduous central incisors and mandibular deciduous molar were most prone to decay in 5,030 children. The prevalence of dental caries was 54.08% (2,720/5,030). The mean dmft score was 2.58 and the filling rate was 0.98% (127/12,993). The prevalence of dental caries and mean dmft increased gradually as the children were growing up (P < 0.05). The rate of filling teeth of the 6 year old group was higher than other groups (P < 0.05). No significant difference between males and females were noted (P > 0.05). The prevalence rate of deciduous dental caries among private kindergartens was 61.04% (1,656/2,713), with a mean dmft of 3.12 and filling rate of 0.35% (30/8,465). The prevalence rate of deciduous dental caries among public kindergartens was 45.92% (1,064/2,317), with a mean dmft of 1.95 and filling rate of 2.14% (97/4,528). Statistical analysis revealed significant differences in the prevalence rate, mean dmft, and filling rate between two kinds of kindergarten (P < 0.05).</p><p><b>CONCLUSION</b>Deciduous dental caries of preschool children is an important problem in Chongqing city and the children from private kindergarten had very low filling rate.</p>


Assuntos
Criança , Pré-Escolar , Feminino , Humanos , Masculino , China , Índice CPO , Cárie Dentária , Cavidade Pulpar , Incisivo , Dente Molar , Saúde Bucal , Prevalência , Dente Decíduo
7.
Artigo em Chinês | WPRIM | ID: wpr-336397

RESUMO

<p><b>OBJECTIVE</b>To investigate influence of oral diseases on child oral health-related quality of life, and to identify the dimensional structure of the early childhood oral health impact scale (ECOHIS).</p><p><b>METHODS</b>The Chinese language version of ECOHIS was applied to 280 patients aged 2-5 years old from the paediatric dentistry clinic of West China School of Stomatology, Sichuan University. The three-dimensional construct was confirmed by confirmatory factor analysis(CFA).</p><p><b>RESULTS</b>Two hundred and sixty-eight valid questionnaires (95.7%) were reclaimed. For the child impact section of the ECOHIS, "pain in the teeth, mouth or jaws" was the most frequently reported item by the parents. Item of "feeling upset or guilty" was most frequently reported in the family impact section of the ECOHIS. The CFA results indicated that all factor loadings to its items were 0.25-0.81 with one factor loadings less than 0.30. The modified factor model revealed a better fit to the data.</p><p><b>CONCLUSION</b>Oral diseases have negative impact on the children's oral health-related quality of life. ECOHIS is a three-dimensional structure.</p>


Assuntos
Pré-Escolar , Feminino , Humanos , Masculino , China , Saúde Bucal , Psicometria , Qualidade de Vida , Inquéritos e Questionários
8.
Artigo em Chinês | WPRIM | ID: wpr-427700

RESUMO

ObjectiveTo evaluate the theoretical knowledge level of Chinese students majoring in stomatology based on the problem-based learning (PBL).MethodsThe Cochrane reviewer's handbook was followed by.Domestic published articles from 1989 -2010 were selected by computer and handwork.All these retrieved studies were controlled clinical trials related to oral theoretical education and were conducted by PBL and the traditional teaching methods.The quality of included studies was critically evaluated and the data were analyzed by the RevMan 5.0.1 software.ResultsA total of 138 articles were selected,but only 8 were included.Significant differences were found when the data were pooled(P < 0.01,I2 =80% ) and random effect model was used for meta-analysis.The results showed that comparing with the traditional teaching methods,PBL can improve the theoretical score of dental students [ MD 5.39,95% CI(2.20,8.58)],and sensitivity analysis showed that the result was stable.ConclusionPBL can improve the theoretical score of Chinese students majoring in stomatology.However,as part of the studies are not high quality literature,the above conclusion needs to be verified by more large sample-sized,rigorously designed randomized controlled clinical trials.

9.
Artigo em Chinês | WPRIM | ID: wpr-241825

RESUMO

<p><b>OBJECTIVE</b>To investigate caries status of primary and permanent teeth among 6-year-old children in Sichuan Province and correlation between caries of deciduous molars and first permanent molars.</p><p><b>METHODS</b>Referenced to the sampling programme of the third national epidemiological survey of oral health status, a total of 714 children aged 6 were randomly sampled in clusters including 3 urban sampling spots and 3 rural sampling spots, the correlation between caries of deciduous molars and first permanent molars was explored.</p><p><b>RESULTS</b>The prevalence rate of deciduous tooth caries of 6-year-old children in Sichuan province was 74.37%, the mean number of dmft of 6-year-old was 3.94 +/- 3.93, the filling rate was only 4.96%. The prevalence rate and mean dmft of deciduous tooth caries showed no significant difference between different area and gender (P>0.05), however, the children came from city showed significant higher filling rate than those from countryside (P<0.05). The first permanent molars erupted have 2.98% in caries, the filling rate was 7.69%. Caries of deciduous molars was the first permanent molars caries risk factors among 6-year-old children in Sichuan province [P=0.001, r=0.175, OR=5.756, 95%CI (1.733, 19.121)]. There was weak correlation between caries of deciduous molars and first permanent molars (P<0.001, r=0.194).</p><p><b>CONCLUSION</b>Caries status of primary among 6-year-old children in Sichuan Province are serious. Caries experience of deciduous molars may play an important role in predicting the first permanent molars caries in clinic and prevention.</p>


Assuntos
Criança , Feminino , Humanos , Masculino , Índice CPO , Cárie Dentária , Dente Molar , Prevalência , Fatores de Risco , Dente Decíduo
10.
Artigo em Chinês | WPRIM | ID: wpr-671027

RESUMO

Objective: To evaluate the effectiveness of laser therapy in the treatment of dentin hypersensitivity in China.Methods: The Cochrane reviewer's handbook was followed.Domestic published articles from 1989~2009 were selected by computer and handsearch.All these retrieved study were clinical control trials related to laser therapy in the treatment of dentin hypersensitivity.The quality of included studies was critically evaluated and data were analyzed by the RevMan 5.0.1 software.Results: A tota1 of 114 articles were selected,but only 9 were included.The results showed that: although the data of immediate efficiency of laser treating dentin hypersensitivity could be combined,significant heterogeneity was found when the other studies' data were pooled.So the data could not be merged,only the descriptive analysis could be used.The immediate efficiency for the treatment of dentine hypersensitivity using lasers was higher than the control group,the difference was statistically significant[OR=3.83,95%CI(2.86,5.12)],other period of efficacy had a trend which was superior to the control group.Conclusion: Comparing with the control group,the desensitizing of dentin hypersensitivity with laser is more effective.However,because the literature quality of this study is not high,the above conclusion needs to be verified by larger sample-size,rigorously designed,randomized controlled clinical trials.

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