RESUMO
Objective:To establish an HPLC method with post-column derivation for the determination of aflatoxin B1 in edible vegetable oil. Methods:An advanced biotechnology-immunoaffinity column was used for the extraction of aflatoxin Bl from the samples, and an HPLC method with post-column derivation was applied to detect aflatoxin Bl in edible vegetable oil, and the results were com-pared with those of the national standard thin layer fluorescence method. Results:The linear range of aflatoxin Bl was 10. 2-51. 0 ng · ml-1(r=0. 9996), the average recovery was 87. 3%(RSD=0. 96%, n=6), and the detection limit was 1 μg · kg-1. Conclu-sion:The method is simple, rapid and sensitive, which can be used as a promoted conventional method for the detection of a large number of samples.
RESUMO
OBJECTIVE: To optimize the sample treatment method in the content determination of gentamycin sulfate sustained-release tablets.METHODS: In the light of the characteristics of the preparation technology of sustained-release tablets,the contents of the same sample treated by 4 methods were compared.Method 1 was a method adopted from Chinese Pharmacopeia;The solution time was increased differently in method 2 and method 3,and method 4 was similar to method 3,but in which additional grinding treatment was performed.RESULTS: Compared with marked value,the approaching degree by method 4 was the highest,followed by method 3,method 2 and method 1,respectively.The average recovery rates for method 1 and method 4 were 91.1%(RSD=1.46%) and 99.8%(RSD=0.74%),respectively.CONCLUSION: Method 4 is the optimal one for sample treatment and it is suitable for the determination of the content of this preparation.