Effect of γ-lactones and γ-lactams compounds on Streptococcus mutans biofilms

Abstract Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 μg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.

Resolução de caso estético complexo por meio de expansão óssea, manipulação tecidual e reabilitação protética com componente protético angulável em tempo real / Resolution of an esthetic complex case through bone expansion, soft tissue manipulation and prosthetic rehabilitation using real-time customized abutments

O objetivo deste trabalho foi apresentar um caso clínico de reabilitação entre dentes e implantes. Na situação inicial, havia escurecimento do dente 11 e ausência do dente 22, sobre o qual estava instalada uma prótese parcial removível. Uma intensa reabsorção óssea na região do dente ausente foi observada através de análises clínica e tomográfica. A técnica de expansão óssea associada ao deslocamento vestibular de tecido conjuntivo palatino, conhecido como "técnica do rolo", foi realizada, juntamente à instalação de implante cone-morse (Arcsys). Após quatro meses, foi realizada a reabertura. Selecionou-se um munhão universal angulável (Arcsys), o qual teve sua angulação personalizada com base na posição obtida através de um referenciador angular, e uma prótese provisória cimentada foi confeccionada sobre este. Um preparo para coroa metalocerâmica foi realizado no dente 11 e sobre o elemento 21, além da substituição das restaurações antigas, também foi realizada faceta em resina composta. Assim, o caso foi finalizado com uma coroa metalocerâmica sobre implante e uma coroa metalocerâmica sobre dente, entremeadas por dente natural facetado com resina composta. Apesar da complexidade de um caso clínico envolvendo dentes naturais e próteses, foi possível alcançar um resultado satisfatório e um sorriso esteticamente harmônico.

The aim of this paper was to present a clinical rehabilitation case with teeth and implants. In the clinical scenario, there was darkening of tooth 11 and the lack of tooth 22, where a removable prosthesis was installed. Also, considerable bone resorption was observed after clinical and CBCT exams. In this way, bone expansion technique was associated to the roll technique. Four months after implant placement, (morse taper, Arcsys), the opening procedure was performed. A universal abutment (Arcsys) was selected and angled in real time according a pre-fabricated guiding device, receiving a cemented provisional single-tooth crown. Then, tooth 11 received a dental preparation for fused-to-metal crown, and tooth 21 received a direct composite veneer. Thus, the final scenario was composed by a fused-to-metal crowns over dental implant and tooth, and a natural tooth with resin composite veneer. Despite its complexity, satisfactory results and a harmonious smile were achieved.