RESUMO
The radioprotective effects of naturally occurring compounds have been investigated in vitro and in vivo considering their pharmacological role in prevention and treatment of cancer. Chitosan (CS) is a naturally occurring polymer that has been increasing attention in pharmaceutical and biomedical applications because of its biocompatibility, biodegradability, nontoxicity, cationic properties and bio adhesive characters. Lymphocytes were treated with different concentrations of chitosan for the period of 2 and 24 hr. Cell viability was determined by tryphan blue dye exclusion assay, single strand DNA damage by alkaline comet assay and in vitro cytogenetic damages were evaluated by micronucleus assays. Treatment of lymphocytes with chitosan before and after the exposure to 4Gy of electron beam radiation (EBR) resulted in the reduction of percentage of tail DNA in comet from 24.06±3.92 to 6.94±1.34 and olive tail moment (OTM) was reduced from 25.34±3.09 to 10.66±0.23 at 10μg/mL concentration. The micronucleus formation in radiation control group (13.75±0.37) was significantly reduced in chitosan pretreated groups 7.63±1.02. Cells treated with chitosan at 10μg/mL showed maximum viability after exposure to EBR. Present investigation data proves the protective effect of CS against EBR induced damage in lymphocyte. However, increase in concentration above 100 μg/mL though resulted in higher protection, an increased cell toxicity was also noticed.
RESUMO
Background: Periodontal disease is an immune-inflammatory disease characterized by connective tissue breakdown, loss of attachment, and alveolar bone resorption. Under normal physiological conditions, a dynamic equilibrium is maintained between the reactive oxygen species (ROS) and antioxidant defense capacity. Oxidative stress occurs when this equilibrium shifts in favor of ROS. Oxidative stress is thought to play a causative role in the pathogenesis of periodontal diseases. Aim: The present study was designed to estimate and compare the superoxide dismutase (SOD) and glutathione (GSH) levels in the serum of periodontitis, gingivitis, and healthy individuals before and after nonsurgical periodontal therapy. Materials and Methods: The present study was conducted in the Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore. The study was designed as a single blinded interventional study comprising 75 subjects, inclusive of both sexes and divided into three groups of 25 patients each. Patients were categorized into chronic periodontitis, gingivitis, and healthy. The severity of inflammation was assessed using gingival index and pocket probing depth. Biochemical analysis was done to estimate the SOD and GSH levels before and after nonsurgical periodontal therapy. Results obtained were then statistically analyzed using ANOVA test and paired t-test. Results: The results showed a higher level of serum SOD and GSH in the healthy group compared to the other groups. The difference was found to be statistically significant (P < 0.0001). The post-treatment levels of SOD were statistically higher than the pre-treatment levels in periodontitis and gingivitis group.