RESUMO
Objective:To investigate the effect of cyclopamine on growth and proliferation of human mammary carcinoma cell line Bcap-37.Methods:The Bcap-37 cells were incubated with cyclopamine,which is the specific inhibitor of Hedgehog signaling pathway.Then the inverted microscope was used to observe the morphologic changes of the cells,and MTT assay,BrdU incorporation and cell cycle analysis were used to examine the influence of cyclopamine on Bcap-37 cell growth and DNA synthesis.Results:Compared with the control group,after the cells were incubated with cyclopamine,obvious morphologic changes of Bcap-37 cells were observed;the growth of Bcap-37 cell was inhibited in a dose and time dependent manner;the DNA synthesis of Bcap-37 cells was obviously inhibited;the number of Bcap-37 cells in S phase decreased and the cells were blocked in G2 phase.Conclusion:Cyclopamine can effectually inhibit the growth and proliferation of Bcap-37 cells,which indicates that Hedgehog signaling pathway may be inappropriately activated in breast cancer and inhibiting Hedgehog signaling pathway can be a useful method in breast cancer treatment.
RESUMO
Objective To investigate the effects of compound nutrients on serum concentrations of IL-6 induced by immobilization and cold water-immersion in rats.Methods Stress was produced by immobilization and cold water-immersion.Rats were randomly as- signed to two groups,i.e.acute and chronic stress groups.Acute stress group was divided again into normal control group(C),acute stress group(AS)and acute stress+compound nutrients group(AS+CN).Chronic stress group was assigned again into normal control group(C),chronic stress group(CS)and chronic stress+compound nutrients group(CS+CN).The serum IL-6 level was determined by enzyme-linked immunosorbent assay(ELISA).Results Oral(gavage)administration of compound nutrients may attenuate effect of stress induced by acute and chronic immobilization,and reduce the increase in serum IL-6 level induced by cold water-immersion stress.In acute immobilization and cold water-immersed stress group,the serum IL-6 level rose significantly from 17.69?0.96 to 29.67?1.64pg/ml 30 minutes after stress,and the administration of compound nutrients effectively attenuated the rise in serum IL-6 level 30 minutes after stress from 29.67?1.64 to 22.30?2.52pg/ml (P