Assessment of experimental saccular aneurysm using selective angiography in common carotid artery of rabbits / 대한해부학회지

In order to study the treatment of aneurysms, the technique of making experimental aneurysms in laboratory animals must be established. In our study, to examine the feasibility of making experimental aneurysm and selective angiography on the common carotid artery in rabbits and to determine the size of experimental aneurysm after surgery, saccular aneurysms were fashioned on the right common carotid artery in 17 rabbits using a vein pouch technique. Selective angiography of the common carotid artery was performed immediately after surgery, and at 1 week, 4 weeks, and 8 weeks after surgery. Also, histological changes in the aneurysms were observed. In 16 rabbits with established successful experimental aneurysm, no differences were found in diet intake and behavior before and after surgery. The patency of the carotid artery was confirmed by selective angiography. The average size of the aneurysm immediately after surgery was similar to that of 1 week postoperatively in selective angiography, however it increased with time at 4weeks and 8 weeks. Histologically, infiltration of inflammatory cells and hemorrhage were found at the junction of the carotid artery and the vein pouch at 1 week, which disappeared at 4 weeks and 8 weeks. This study suggests experimental saccular aneurysm using the vein pouch technique might form aneurysms similar to that of the human in its properties such as increment of size, and selective angiography might be suitable for assessment of experimental aneurysm. Therefore, this animal model may be suitable for investigating new treatment methodologies for human aneurysms.

Application of a temporary palatal prosthesis in a puppy suffering from cleft palate

A 3-month-old Schnauzer was presented with congenital defects of the secondary palate. On the clinical examination, coughing, sneezing, drainage of nasal discharge from the external nares and poor growth were found. Vital signs and results of blood examination were within normal ranges. Thoracic radiography revealed mild pneumonia in the right lung lobes. In a puppy suffering from cleft palates, a palatal prosthesis was applied to the hard palate in order to protect the surgical wound, because a routine surgery was not successful. A palatal prosthesis was applied and held in place using the instant glue and plastic bands to protect the surgical wound following the third repeated surgery. Although a small oronasal fistula still remained, there was no functional defect. This prosthesis was easy to apply and helpful to protect the surgical wound. In addition, this implant could be placed or adjusted without or sedation/anesthesia.

Immobilization with Ketamine HCl and Tiletamine-Zolazepam in Cynomolgus Monkeys

To compare the effects of ketamine and tiletaminezolazepam (TZ) drugs widely used for the chemical restraint and immobilization of primates, on various physiological parameters and blood gas values in cynomolgus monkeys (Macaca facicularis). Rectal temperature, heart rate, respiration rate and blood gas analysis were measured before treatment and at 1, 10, 20, 30, 40, 50 and 60 min after administration. Additionally, in both groups, induction and maintenance times were compared. Heart rate, respiration rate, rectal temperature, pH and pCO2 were not significant different in the two groups. However, pO2 in the ketamine-treated group was significantly lower at 30 and 40 min than in the TZ-treated group. The induction time was short in both groups, and the maintenance time was longer in the TZ-treated group (67.8+/-6.5 min) than in the ketamine-treated group (42.3+/-6.7 min). However, decreased rectal temperatures must be watched and prevented following TZ administration to cynomolgus monkeys. It was considered that ketamine may be useful for short duration anesthesia including handling, physical examination, blood sampling and TZ may be useful for prolonged anesthesia including minor surgery and other surgical procedure.