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1.
Journal of the Korean Society for Microbiology ; : 389-399, 1997.
Artigo em Coreano | WPRIM | ID: wpr-81358

RESUMO

Mobility shifts in non-denatured gel electrophoresis of PCR-amplified Rif' region in each of fifteen different mutants of M. tuberculosis were discerned by single strand conformation polymorphism (SSCP) analysis. The findings of mobility differences between rifampin-resistant and susceptible strains showed an excellent agreement with data obtained by traditional susceptibility test. SSCP-PCR seemed to replace the cultivation method of susceptibility test that was known to be time-consuming, labor wasting, and skeptical in quality control. After screening of rpoB gene mutation by SSCP-PCR, detection of specific sequence changes in the region of rpoB gene was attempted through the procedures of PCR-amplification, cloning of PCR-products using pGEM-T vector and DNA thermocycling sequencing. Fifteen different types of mutations were identified among fifty strains of rifampin-resistant strains while five rifampin-susceptible control strains showed no sequence changes of rpoB gene as well as reference strain H37rv. Most mutation appeared to be a point mutation due to substitution or deletion except seven mutants showing somewhat complex mutation. Each of mut#ated loci inclined to clustering within a region of eighteen amino acids involving eight codons. The most common mutation of Ser425 shared among twenty-nine mutants and followed by eleven mutants of His420. Several mutants alleles identified in this study appeared to be dissimilar to those of previous reports.


Assuntos
Alelos , Substituição de Aminoácidos , Aminoácidos , Células Clonais , Clonagem de Organismos , Códon , DNA , Eletroforese , Programas de Rastreamento , Mycobacterium tuberculosis , Mycobacterium , Mutação Puntual , Controle de Qualidade , Rifampina , Tuberculose
2.
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 561-566, 1991.
Artigo em Coreano | WPRIM | ID: wpr-649668

RESUMO

No abstract available.

3.
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 263-270, 1991.
Artigo em Coreano | WPRIM | ID: wpr-645882

RESUMO

No abstract available.


Assuntos
Sinusite
4.
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 1225-1231, 1991.
Artigo em Coreano | WPRIM | ID: wpr-651137

RESUMO

No abstract available.


Assuntos
Rinorreia de Líquido Cefalorraquidiano , Líquido Cefalorraquidiano
5.
Korean Journal of Otolaryngology - Head and Neck Surgery ; : 359-365, 1991.
Artigo em Coreano | WPRIM | ID: wpr-654313

RESUMO

No abstract available.


Assuntos
Pneumocefalia
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