Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment

The aims of this study were to investigate the utility of solid microneedle arrays (150 µm in length) in enhancing transdermal delivery of peptides and to examine the relationship between peptide permeation rates and D2O flux. Four model peptides were used (Gly-Gln-Pro-Arg [tetrapeptide-3, 456.6 Da], Val-Gly-Val-Ala-Pro-Gly [hexapeptide, 498.6 Da], AC-Glu-Glu-Met-Gln-Arg-Arg-NH2 [acetyl hexapeptide-3, 889 Da] and Cys-Tyr-Ile-Gln-Asn-Cys-Pro-Leu-Gly-NH2 [oxytocin, 1007.2 Da]). The influence of microneedle pretreatment on skin permeation was evaluated using porcine ear skin with Franze diffusion cell. Peptide permeation across the skin was significantly enhanced by microneedle pretreatment, and permeation rates were dependent on peptide molecular weights. A positive correlation between D2O flux and acetyl hexapeptide-3 clearances suggests that convective solvent flow contributes to the enhanced transdermal peptide delivery. It is concluded that solid microneedle arrays are effective devices to enhance skin delivery of peptides.

Clinical efficacy and safety analysis of left colon and intestinal surgery phase I and phase II / 中国基层医药

Objective To analyze clinical efficacy and safety of left colon and bowel obstruction .Methods 80 cases of left colon and bowel obstruction were divided into the observation group and control group,40 cases in each group.The observation group was given a surgery,and the control group received two surgical treatment,the two groups were taken the statistical analysis of clinical data to compare the clinical efficacy and safety of operation. Results The operative time,hospital stay and surgical blood loss in the observation group was (42.9 ±13.8) min, (9.3 ±2.8)d,(45.9 ±11.7)mL,which in the control group were (58.6 ±17.6)min,(6.8 ±1.5)d,(151.4 ± 19.4)mL,there were statistically significant difference between the two groups(t=25.358,16.128,29.475,all P<0.05);The abdominal abscess,lung infections and wound infection incidence of adverse reactions in the observation group was 7.5 %,10.0%,20.0 %,which in the control group was 17.5 %,2.5%,10.0%,there were statistically significant difference between the two groups (t=4.512,4.336,4.762,all P<0.05).Conclusion The clinical treatment of left colon and bowel obstruction shall promptly conduct a surgery,the role of hospitalization in patients with shorter length to enhance tumor resection rate,and actively improve the quality of life of patients.