Vectors of Japanese encephalitis virus (JEV): species complexes of the vectors.

The vectors of JEV are Cx. tritaeniorhynchus, Cx. vishnui, Cx. pseudovishnui, Cx. gelidus, Cx. fuscocephala, Cx. quinquefasciatus, Cx. pipiens pallens, Cx. bitaeniorhynchus, Cx. annulirostris, Aedes togoi, Ae. japonicus, Ae. vexans nipponii, Anopheles annularis and An. vagus. Cx. tritaeniorhynchus is in the tritaeniorhynchus complex, breeds in rice fields, ground pools in vast areas. Two types of mating behavior, eurygamy and moderate stenogamy were detected. In the case of the eurygamy type, the mosquitoes were from Southern Thailand and hilly areas near Kanchanaburi, Thailand. Female mosquitoes are usually dark in color, the cibarial armature has rod teeth and the posterior end of the cibarial armature is bowl shaped with a typical rim. The rim of the bowl is everted. The moderate stenogamy type were mosquitoes from the plain areas such as Bangkok, Ayutthaya, Suphan Buri and Saraburi. The posterior end of the cibarial armature is bowl shaped with a stout rim. The larvae were characteristic in their siphon index, antennal index, hair O of prothoracic segment, and comb scale number and arrangement. Cx. tritaeniorhynchus summorosus from Japan, Los Banos and Luzon, Philippines, differed from Cx. tritaeniorhynchus in that on the lateral plate of the phallosome tritaeniorhynchus teeth are somewhat weakly developed and only gently curved whereas in tritaeniorhynchus summorosus they are strongly developed, considerably longer, and sharply recurved. The siphons of larvae are short, the sides parallel and the apex truncate in tritaeniorhynchus whereas in tritaeniorhynchus summorosus they are long and slender. Cx. tritaeniorhynchus var. siamensis is possibly present. Colonies have been maintained in the Department of Medical Entomology for 31 generations. The characteristics are in hair O (short, less than 20 branches, and without secondary branching and the larval siphon (short and broad where the others are long). Cx. vishnui and Cx. pseudovishnui are in the vishnui complex. Cx. quinquefasciatus and Cx. pipiens pallens are in the Cx. pipiens complex comprising: (1) Cx. pipiens; (2) Cx. quinquefasciatus Say; (3) Cx. molestus Forskal; (4) Cx. pipiens pallens; (5) Cx. australicus; (6) Cx. globocoxitus. Anopheles annularis is a species complex evidenced by two types of polytene chromosomes.

A brief survey of phlebotomine sandflies in Thailand.

Sandflies were found in widely scattered localities in Thailand in varied numbers. Daytime resting places for the adults included caves, termite hills, abandoned houses, ancient stone sanctuaries, air-raid shelters, tree hollows, loose bark of dead standing trees and rock crevices. Of fifteen species, P. major major, P. teshi, S. anodontis, S. gemmea, S. hodgsoni hodgsoni, S. perturbans, S. punjabensis and N. vietnamensis were here recorded for the first time in Thailand. P. argentipes and P. major major are interesting in view of their potential as disease vectors.

Observations on the feeding habits of four species of Mansonia (Mansonioides) mosquitoes in Southern Thailand.

Studies on feeding habits of Mansonia annulata, M. annulifera, M. indiana and M. uniformis were carried out in southern Thailand. This study showed that M. annulata and M. uniformis were both strongly exophagic mosquitoes which avoided approaching and entering human dwellings. The biting frequency on the verandah was considerably higher than indoors. M. indiana was not repelled by houses but avoided biting indoors. M. annulifera was endophagic, it was attracted by houses and bit on the verandah as frequently as indoors. Some species showed differential preference for age- and sex-groups of human hosts: M. annulata was more attracted by adolescents and adults than by children and M. annulifera preferred female hosts. The other species did not distinguish between host groups.

Some aspects on biting cycles of Culex quinquefasciatus in Bangkok.

The indoor and outdoor biting cycles of Culex quinquefasciatus were studied in Bangkok. The biting cycle was nocturnally periodic and preferably endophilic in character. There were two minor peaks on top of the nocturnally periodic peak. The first peak was between 2200 and 2300 hours, the latter peak was after midnight being at 0100 and 0400 hours. Parous mosquitoes formed 0%-20.0% of hourly total population. More parous mosquitoes were collected indoor and also during in the latter half of the night. Comparisons made between Bangkok, the none endemic area for Wuchereria bancrofti and Sri Lanka the endemic area, revealed that Bangkok has a lower survival rate of Culex quinquefasciatus, based on parous rates and that the older mosquitoes, which are potentially infective vectors were collected late in the night. This evidence could explain why Bangkok is not endemic area for periodic Wuchereria bancrofti.

Effect of a single dose of primaquine on a Thai strain of Plasmodium falciparum.

A single dose of 45 mg of primaquine base was effective in the elimination of gametocytes of the possibly chloroquine-resistant P. falciparum in Thailand. The gametocyte counts decreased markedly within 3.5 days showing significantly difference from the control in which mostly gametocytes still remain within 11.4 days. The development of the gametocytes in A. dirus was not observed after treatment. Primaquine can be used as gametocytocide and sporontocide for falciparum malaria in Thailand if the drug is administered when matured gametocytes appeared in the peripheral blood.

The vectors of Brugia malayi in Southern Thailand.

Mansonia uniformis, with an infective rate of 0.02, was incriminated as the vector of periodic Brugia malayi in Pattani province. Mansonia bonneae and Ma. dives, with infective rates of 0.18 and 0.20 respectively, were the vectors of B. malayi in Narathiwat, where the microfilarial periodicity was the subperiodic form.