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Objective:To explore the effects of antigen-specific IgG in immunologic mechanism of bronchial asthma.Methods:A experimental mouse model of bronchial asthma with ventilating function being the determinative norm of bronchial asthma was duplicated.Then,antigen-specific IgG,IgE in the mice’s sera were detected by method of indirect enzyme linked immunosorbent assay,and the effects of IgG in bronchial asthma were analyzed preliminarily by test of linear correlation between IgG and airway resistance.Results:The level of antigen-specific IgG ( t =4.537, P
RESUMO
AIM: To further investigate the mechanism of bronchial asthma.METHODS: Experimental asthma mouse model was established with OVA, and immunocytes were isolated, including macrophages, dendritic cells, B cells, CD3 +T cells, CD4 +T cells and CD8 +T cells from spleen. Antibody dependent cell-mediated cytotoxicity(ADCC) effects of all these cells were detected with cell proliferation and improved MTT colorimetry methods.RESULTS: The ADCC functions of M, B cells, CD3 +T cells,CD4 +T cells and CD8 +T cells from asthmatic mice were weaker than that from control ( P