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Objective To investigate the incidence and risk factors of neonatal asphyxia in Hubei Enshi Tujia and Miao Autonomous Prefecture. Methods Live born infants, including those diagnosed with neonatal asphyxia, were recruited from 16 different hospitals in Hubei Enshi Tujia and Miao Autonomous Prefecture from January to December of 2016. The 16 hospitals included four grade A tertiary hospitals (three general hospitals and one traditional Chinese medicine hospital) and 12 grade A secondary hospitals (eight general hospitals, one maternal and child health hospital and three traditional Chinese medicine hospitals). A retrospective investigation was conducted using questionnaire to analyze the basic information, perinatal risk factors and prognosis of those infants. Chi-square test was used for statistical analysis. Results Among 22 294 recruited live born infants, 733 (3.29%) were diagnosed with neonatal asphyxia on discharge, including 627 (85.54%) mild cases and 106 (14.46%) severe cases. And neonatal asphyxia resulted in deaths of 27 cases (3.68%). The risk factors for neonatal asphyxia included multiple pregnancy, pregnancy conceived with assisted reproductive technology, premature infant, low birth weight infant, fetal malposition, congenital malformation, male infant, born during transfer, mother of Tujia nationality, low educational level (primary school or lower), living in rural area, the number of antenatal visits ≤3, history of early threatened abortion, anemia in pregnancy, hypertensive disorders of pregnancy, chorioamnionitis, abnormal pregnancy history and abnormality of umbilical cord, amniotic fluid or placenta. Conclusions The incidence of neonatal asphyxia in Enshi area is obviously higher than the national average. The main risk factors for neonatal asphyxia in this area are related to maternal background and the living condition of the mother during pregnancy, delivery as well as the newborn at birth.
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Objective To investigate the differences in expression profile of long non-coding RNA (lncRNA) in retina between normal newborn mice and those with oxygen-induced retinopathy (OIR) to lay a foundation for further study of regulatory mechanisms of lncRNA in retinal neovascularization, and to provide a new theoretical basis for the prevention and treatment of retinopathy of prematurity (ROP). Methods A total of 48 cleaning grade C57BL/6J neonatal mice were randomly divided into two groups with 24 in each: OIR group and control group. Those in the OIR group were exposed to hyperoxia [(75±2)％ O2] from 7th to 12th day after birth, and then re-exposed to normoxia (room air) for five days to establish a OIR mouse model. Mice in the control group were raised in room air all the time. Retinal tissues were collected from each mouse on the 17th day after birth. Retinal angiography and hematoxylin-eosin (HE) staining were performed to confirm the establishment of OIR mouse model. Expression profiles of lncRNAs were analyzed by lncRNA high-throughput sequencing and the results were verified by real-time fluorescence quantitative polymerase chain reaction (PCR). Based on the expression of lncRNAs and mRNA and the relationship between their genomic locations, potential target genes of lncRNAs were obtained. Gene ontology analysis (GO) and Kyoto Encyclopedia of Genes and Gnomes (KEGG) pathway analysis were used for bioinformatics analysis of lncRNA expression profile in OIR. T test and Graphpad prism were used for statistical analysis and spreadsheet. Results Retinal angiography and HE staining identified serious retinal damage in the OIR group. In total, 1118 differentially expressed lncRNAs ( ≥ 2.0-fold difference in expression, P<0.05) relating to OIR between the two groups were identified by lncRNA high-throughput sequencing. Results of real-time fluorescence quantitative PCR confirmed that XLOC_150632 and XLOC_150636 were upregulated in the OIR group as compared with those in the control, while XLOC_122045,XLOC_100454, XLOC_170009 and XLOC_122042 were downregulated. GO analysis showed that 852, 1148 and 2100 target genes of differentially expressed lncRNAs were relating to biological process, cellular component and molecular function, respectively. KEGG analysis showed that the target genes were associated with 36 biological pathways, mainly relating to retinal development, chemotaxis, migration and energy metabolism of vascular endothelial cells. Conclusions lncRNAs are differentially expressed in healthy newborn mice and newborn mice with OIR. Fibroblast growth factor 2 (FGF2), the potential target gene of XLOC_150632 and XLOC_150636, is related to phosphatidylinositol-3-kinase (PI3K) signaling pathway and may be involved in retinal neovascularization. This might be a potential target for ROP prevention and treatment.
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Objective To analyze the characteristics and correlated factors of attempted suicide among psychological aid hotline callers,and provide a reference for suicide prevention and intervention to the domestic psychological aid hotlines.Methods From September,2010 to August,2015,1 299 callers who had the database of attempted suicide among hotline callers were collected.During the calling,the callers were interviewed on their attempted suicides,depression and 10 correlated factors associated with caller's suicidal behaviors.Results Among 1 299 callers who received assessment of suicidal behavior relates factors,361 (27.8％) had evaluated of current suicidal ideation or behavior,223 (17.2％) had history of prior suicidal attempts.There was no significant difference in the incidence of suicide attempts among different gender,age and occupation.1 024 (78.8％) had symptoms of mental illness,794 (61.1％) had symptoms of anxiety,708 (54.5％) had symptoms of depression,864(66.5％) had concomitant two or more symptoms.Compared the caller's occurrence rate of suicidal attempter between single depression and concomitant other symptoms,the results found single depression and concomitant anxiety,concomitant obsession,concomitant panic and concomitant fear were statistically significant.Multiple logistic regression analysis showed that high depression emotion (β=O.389,OR=1.48,95％CI=1.08-2.02),the history of prior attempted suicide (3=0.487,OR=1.63,95％CI=1.19-2.22) and high hopelessness (β=0.618,OR=1.86,95％CI=1.32-2.60) were associated with the caller of ever attempted suicide behaviors.Conclusion Among the callers with attempted suicide,in order to improve the effectiveness of hotline prevention and invention,the psychological consultant should focus on the evaluation of concomitant mental symptoms,high depression emotion,history of prior attempted suicide and hopelessness.
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Objective To characterize hotline callers and related factors in Xiamen psychological aids hotline.Methods The data of 8397 callers who had used hotline were recruited from September 2010 to August 2015.During the calling,the callers were interviewed on their demographic characteristics,main counseling problems and suicidal related factors.Results Among the 8397 callers,1304 (15.5％) were frequent callers.Frequent callers had a high proportion of love and interpersonal problems,learning problems,mental illness problems.After adjusted for demographic characteristics,suicidal ideation or behavior (OR=1.417,95％CI:1.083～1.853),prior suicidal behavior 2 weeks before calling (OR=1.453,95％CI:1.058～1.996),symptoms of mental illness (OR=1.909,95％CI:1.188～3.065) were associated with repeated calls among hotline caller.Conclusion Counselor should pay attention to the suicidal related factors in psychological aids hotline to provide better service for hotline caller.
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Objective To characterize hotline callers and related factors in Xiamen psychological aids hotline.Methods The data of 8397 callers who had used hotline were recruited from September 2010 to August 2015.During the calling,the callers were interviewed on their demographic characteristics,main counseling problems and suicidal related factors.Results Among the 8397 callers,1304 (15.5％) were frequent callers.Frequent callers had a high proportion of love and interpersonal problems,learning problems,mental illness problems.After adjusted for demographic characteristics,suicidal ideation or behavior (OR=1.417,95％CI:1.083～1.853),prior suicidal behavior 2 weeks before calling (OR=1.453,95％CI:1.058～1.996),symptoms of mental illness (OR=1.909,95％CI:1.188～3.065) were associated with repeated calls among hotline caller.Conclusion Counselor should pay attention to the suicidal related factors in psychological aids hotline to provide better service for hotline caller.
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Objective:To investigate the levels of serum leptin and their effect on Th 1/Th2 balance and the association between serum leptin levels and clinical manifestations in obese children with asthma.Methods: All 56 children with physician-diagnosed moderate-to-severe persistent asthma and 56 healthy controls were included.The children with asthma and without asthma were divided into two groups,obese (n=28) and non-obese (n=28) by body mass index,respectively.Asthma severity was assessed by the asthma symptom score.Peripheral blood samples were collected to determine the levels of leptin , IFN-gamma and IL-4.The results were correlated with the parameters studied.Results:Serum leptin levels were significantly higher in the obese asthma group than in the non -obese asthma group ,as well as being significantly higher in the children with asthma than in the controls ( P<0.01 ) , whereas IFN-gamma levels were significantly higher and IL-4 levels were significantly lower in the obese asthma group than in the non-obese asthma group( P<0.01 ).In addition, the obese asthma group showed higher asthma symptom scores and significantly lower FEV 1 (% of predicted) than did the non-obese asthma group(P<0.01).There was a significantly positive correlation between leptin ,IFN-gamma and IL-4 levels only in the obese asthma group ( P<0.01 ).Conclusion:Leptin is involved in the pathogenesis of asthma in obese and non-obese children ,and its effect is more pronounced in the former.In the presence of high leptin levels , only obese children with asthma exhibited Th1 polarization,with higher IFN-gamma levels and greater asthma severity.