RESUMO
A simple and precise RP-HPLC method was developed for simultaneous determination of piroxicam and ofloxacin in pharmaceutical formulations and human serum. Optimum separations of piroxicam, ofloxacin and stress-induced degradation products were achieved by use of Hypersil BDS C8 column [250 x 4.6mm, 5microm]. The mobile phase was a mixture of acetonitrile: 0.012M K[2]HPO[4]: 0.008M sodium citrate [both buffers mixed and pH adjusted to 2.8] [50:25:25 v/v/v] delivered at flow rate of 1.5mL min[-1] using DAD at 254nm. Response was linear function of concentration over the ranges of 70-130mg mL[-1] for piroxicam and ofloxacin [r[2]>/=0.999]. The method efficiently separated the analytical peaks from degradation products with acceptable tailing and resolution. The developed method was successfully used for concurrent analysis of piroxicam and ofloxacin in pharmaceutical formulations, human serum and in vitro drug interaction studies