[ first survey on the status of bovine immunudeficiency virus infection and associated clinical, pathological, haematological and flow cytometric findings in Holestein cattle in Iran]

Bovine Immunodeficiency Virus is a Lentivirus which can cause varieties of infectious complications and induce remarkable losses to dairy industry. Considering to the probability of worldwide distribution of the BIV infection, this study was conducted for the first time in Iran to determine of the status of BIV infection in the Holstein cattle and evaluate the associated findings. According to the results of the Nested-PCR method in this study, 16.2% of 197 slaughtered Holstein cattle in Meysam abattoir of Tehran were infective with BIV. Sequencing of the amplicons obtained from PBLs of the cattle using gag-specific primers revealed that there was 98.7-98.9% homology between this field strain and reference sequences of BIV. The infection rate of cattle with enlarged heamal nodes was higher than the animals without these lesions [0.01

[ abattoir survey on prevalence of enzootic bovine leukosis virus infection and associated clinical, haematological and flow cytometric findings in holestein cattle in Tehran]

Bovine leukosis virus [BLV] is a retrovirus that primarily affects lymphoid tissue of cattle. To determine the seroprevalence of Enzootic Bovine Leukosis Virus infection and its effects on some clinical, hematological and flow cytometric indications, this study was conducted on 197 Holstein cattle slaughtered in an abattoir in Tehran province. ELISA gp5 1 test of the obtained serums of the animals showed that the mean of infection rates was 22.3%. Age arrangement of the relative frequencies of the infection showed a significant correlation between EBLV infection and age increasing [0.001

BVDV induced gastro-neuropathy outbreak in a feedlot calves around Tehran [Iran]

An outbreak of a lethal disease was reported in 4-6-month-old Holstein calves in a feedlot around Tehran. The signs of central nervous system and gastrointestinal system [GI] involvement were observed in the diseased animals. Necropsy samples of GI, liver, kidney, spleen and lung from 3 died animals were prepared for histopathological examination. Blood and formalin-fixed ear notch samples of 6 calves were submitted for RT-PCR, antigen-capture ELISA [ACE] and immunohistochemistry [IHC] for the detection of BVDV. The results of ACE on buffy coats were negative but RT-PCR of all 6 cases and IHC of 4 cases were positive for BVDV infection. Based on the clinical signs and pathological findings in the GI system and brain, we strongly suggest that the BVDV may represent a gastro-neuropathogen strain. To the best of our knowledge, this is the first outbreak of gastro-neuropathogenic BVDV infection in Iran, which may be acquired postnatally

Experimental study on the relationship between nutritional hypophosphatemia and hemoglobinuria in the lambs

This study was designed to induce nutritional hypophosphatemia to investigate the possibility of intervascular hemolysis in the control [7 lambs] and test [8 lambs] groups. Phosphorus of the ration jugular vein blood samples were weekly taken for inorganic phosphorus, alkaline phosphatase, billirubin, hematocrit and Heinz body examination. The independent t-test and repeated measures [analysis of variance] methods applied for the statistical analyses. Significant hypophosphatemia [p<0.01] and increase in the alkaline phosphatase activity [p<0.001] observed in the test lambs. Hienz bodies in the red blood cells and highly significant decrease of the hematocrit [p<0/0005] appeared in both groups. Although, visible hemoglobinuria was not observed .The results cleares up potential sensitivity of sheep to nutritional hemolysis

[ survery on frequency of foot-and-mouth disease virus carriers of slaughtered cattle in Zyaran abattoir]

To determine frequency of FMDV carriers in slaughtered cattle at Zyaran abattoir. Cross-sectional study. Three hundred and one tonsils of clinically normal cattle. All of tonsils were tested for determination of FMDV genome using RT-PCR. Then, 30 samples were cultured on MDBK. 20 samples had positive findings in RT-PCR test. Based on sex, age and breed of cattle, RT-PCR results compared with Chi-square and Fisher's Exact Test. McNemar test was used for comparing of virus culture results with RT-PCR. Ninety nine samples were positive in RT-PCR. Relative frequency of FMDV genome presence in tonsils of clinically normal cattle had a significant difference based on sex and breed. The Sistani and Holstein bred had the highest and the lowest relative frequency, respectively samples on cell serotype O of culture were FmDV. One positive sample [Asia 1] Two positive sample[Asia1] was shown by ELISA. Total relative frequency of positive FMDV genome [32.9%] indicates the extensive exposure to FMDV. Breed variation among Sistani cattle [as a variety of Bos indicus] and other bred should be studied in a controlled condition