Analysis of human urinary proteins after physical exercise by two-dimensional electrophoresis under non-denaturing conditions / 体力科学

A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19-21 years) were loaded physical exercise with a 10km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68-2.56mg/d<I>l</I>), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.

Specific plasma protein detected on physical education athletic students / 体力科学

The purpose of this study is to analyze the plasma proteins of physical education athletic and general students (aged 19-22) before their breakfast by two-dimensional electrophoresis.<BR>The results of this study, a protein which has not been found in any original report yet is detcted from physical education athletic students before breakfast.<BR>This protein was not detected from general studenets before breakfast. This protein was detected at the position of pI5.0, molecular weight of about 70, 000 on two-dimensional polyacrylamide gel electrophosis under non-denaturing codition, but it showed a molecular weight of about 30, 000 on SDS-polyacrylamide gel electrophoresis.<BR>As far as this research is concerned, neither special physical education athletic students and nor general students were observed in changes of two-dimensional electrophoretic patterns of blood cell cytosol and blood cell membrane proteins.