[Simulated microgravity effects on cell cycle in adipose derived stem cells]

Background and Aim: In recent years, biophysical forces especially microgravity increasingly has been used for cell manipulation, in particular cell differentiation and tissue engineering. Microgravity, as a mechanical factor, has been demonstrated to stimulate cell proliferation and differentiation. In this study, we investigated the impact of simulated microgravity condition generated by one-axis clinostat, on cell cycle and apoptosis rate of adipose derived stem cells [ADSCs]

Material and Methods: After isolation and characterization of human ADSCs, they were exposed to simulated microgravity condition generated by one-axis clinostat, for 3 days. After 3 days of clinorotation, control and microgravity samples were stained with PI, then, we evaluated cell cycle and apoptosis rate

Results: Isolated cells expressed mesenchymal markers and were able to differentiate into adipocytes and osteocytes. Our results showed that 3 days of simulated microgravity induced by one-axis clinostat had no statistically significant effects on the cell cycle and apoptosis rate

Conclusion: Previous studies have shown that simulated microgravity induced by 3-axis clinostat or RPM altered cell cycle and increased apoptosis rate. Based on our data, it seems that shear stress generated by one-axis clinostat is much less than that generated by three-axis clinostat. Therefore, we recommend use of one-axis clinostat for evaluation of cell differentiation and cell therapy under microgravity conditions for the future studies

effect of one session low level laser therapy of extracted follicular units on the outcome of hair transplantation

Introduction: Photobiostimulation with low level laser [LLL] has been used in medicine for a long time and its effects have been shown in many diseases. Some studies have evaluated the effect of LLL on androgenic alopecia. One of the most important limitations of the use of LLL in the treatment of alopecia is the requirement for multiple sessions, which is hardly accepted by patients. This study was conducted to evaluate the effect of the irradiation of extracted follicular hair units by LLL on the outcome of hair transplantation

Methods: We enrolled 10 patients with androgenic alopecia and after screening tests for infections and other diseases, we extracted hair follicular units. The hair units were divided in two groups. One group was irradiated by LLL 20 minutes before transplantation [660 nm, 80 Hz, 100 mW] and the other one was used as control. The containing plates were labeled as A and B and sent to the operation room. The surgeon was unaware of the therapy assigned to the plates and transplanted them randomly on the right or left side of the head. One hundred follicular units on each sides of the scalp were transplanted symmetrically. The follicles on both sides were evaluated at 3 and 6 months of transplantation for hair growth rate by another physician, blinded to the treatment assigned to each side

Results: Ten patient with androgenic alopecia and mean [SD] age of 31.5 [6.6] years [range 25-45 years] completed the study. All patients had 100% hair growth at 3 and 6 months follow-up except one who had hair growth of 20% at three months of transplantation, which changed to 100% at sixth months. There was no significant difference between the groups regarding hair growth [P>0.8]

Conclusion: One session of LLL irradiation has no significant effect on the outcome of transplanted hair follicles. Studies with larger sample size are needed to draw a definite conclusion

Coronary artery revascularization in a young adult with solitary coronary aneurysm probably secondary to childhood Kawasaki disease

Surgical revascularization for coronary artery lesions secondary to Kawasaki disease [KD] has been rarely reported in adolescent patients. We report a young adult with no coronary risk factors but with a giant solitary coronary aneurysm with obstructive thrombosis inside, presumably secondary to KD, who underwent coronary artery bypass grafting [CABG] with left internal thoracic artery [LITA] and SVG. Because coronary artery sequelae of KD can be a cause of ischemic heart disease even in young adults, heightened awareness of this entity is required for young adults with coronary lesions but without coronary risk factors

[ effect of lavender a aromatherapy on the pain intensity perception and intrapartum outcomes in primipare]

;Labor pain is one of main preoccupations of pregnant women. In this study, our aim was determining the lavender aromatherapy on pain intensity perception and intrapartum outcome in primipare. The present randomize control trial [RCT] study was conducted at Shiraz University of Medical Sciences in 2007. Qne hundred sixty participants, who enrolled voluntarily in our survey and signed the consent form, were divided into two equal groups by simple sampling. The intervention days were randomly separated to avoid any bias. The aroma group received 0.1 milliliter of lavender essential oil mixed with 1 milliliter of distilled water, via tissues attached to their gown close to their nostrils, and the control group received just 2 cc of distilled water via the same way. Pain perception intensity was measured by visual analogue scale [VAS] before the intervention, 30 and 60 minutes after aromatherapy respectively. Both groups were observed during labor time to monitor any aroma side effects. Collected data were analyzed by t-test and chi-square using the SPSS software. Independent T test analysis revealed that the differences in the means of pain perception intensity before intervention between the two groups are not significant while the means of pain perception intensity in the aroma group was lower than that of the control group at 30 and 60 minutes after the interventions [p<0.001]. Paired T test showed that the means of pain perception intensity in the aroma group did not differ before the intervention 30 and 60 minutes after the intervention, but in the control group, had significant increasing in pain perception intensity [p<0.001] was seen. Aromatherapy with lavender did not affect the duration of labor phases and Apgar score. The present study revealed that aromatherapy with lavender affects pain perception of labor pain in the aroma group, but did not affect duration of labor phases and Apgar score