Evaluation of hypoglycaemic and hypolipidemic activities of Globularin isolated from Globularia alypum L. in normal and streptozotocin-induced diabetic rats.

The present study investigates the effect of intraperitoneal administration of Globularin on blood glucose levels in normal and streptozotocin diabetic rats. Globularin was an iridoid glucoside which was isolated from the leaves of Globularia alypum (3.4 %). This compound was identified by means of physical constants and spectral data UV, IR, MS, 1H-NMR, 13C-NMR, DEPT, COSY, HMBC. The acute toxicity test demonstrated that Globularin is not lethal up to dose of 1000 mg/kg body weight after intraperitoneal injection. In normal and streptozotocin diabetic rats, single intraperitoneal administration of Globularin at a dose 100 mg/kg body weight produced significant decrease of blood glucose levels. However, in prolonged treatment study, the repeated intraperitoneal administration of Globularin (2 x 100 mg/kg body weight) decreased significantly the blood glucose levels when compared to the diabetic control rats. In addition, daily injection of Globularin (2 x 100 mg/kg body weight) reduced significantly serum levels of total cholesterol (varied from 0.53 to 0.41 g/L) and triglycerides (varied from 1.71 to 0.79 g/L) in the diabetic rats.

Preliminary phytochemical screening andantioxidant activities of solvent extracts from Daucus crinitus Desf., from Algeria.

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as primary antioxidant free radical terminators. This paper reports the antioxidative activity of methanolic and water extract of Daucus crinitus Desf. Phytochemical screening of the crude extracts of stems/leaves revealed the presence of different kind of chemical groups such as tannin, flavonoids, phenolic acids and coumarins. The amounts of total phenolics and flavonoids in the solvent extracts (methanol and water extract) were determined spectrometrically. From the analyses, methanolic extract had the highest total phenolic content (130.19 μg GA/mg extract) and antioxidant activity (89.82 %) using DPPH method. Increasing the concentration of the extracts resulted in increased ferric reducing antioxidant power for both extracts tested. Finally, a relationship was observed between the antioxidant activity potential and total phenolic and flavonoid levels of the extract.