RESUMO
Purpose@#The purpose of this study is to identify that emotional labor has effect on depressive symptom, job satisfaction, and moderating effect of self-esteem and ego-resilience of nurses. @*Methods@#Data were collected by questionnaires from 224 nurses university hospitals from May 8 to May 19, 2017, analyzing by hierarchical multiple regression. @*Results@#There was a significant positive correlation with emotional labor and depressive symptom. Also emotional labor was significant negative correlation with job satisfaction, self-esteem, and ego-resilience. Ego-resilience had a moderating effect that emotional labor affected depressive symptom and job satisfaction. @*Conclusion@#In this study, Ego-resilience were found to have a significant moderating effect in relation to emotional labor, depressive symptom, and job satisfaction. Therefore, it's needed to consider the ego-resilience when developing emotional labor management program of nurses.
RESUMO
Purpose@#The purpose of this study is to identify that emotional labor has effect on depressive symptom, job satisfaction, and moderating effect of self-esteem and ego-resilience of nurses. @*Methods@#Data were collected by questionnaires from 224 nurses university hospitals from May 8 to May 19, 2017, analyzing by hierarchical multiple regression. @*Results@#There was a significant positive correlation with emotional labor and depressive symptom. Also emotional labor was significant negative correlation with job satisfaction, self-esteem, and ego-resilience. Ego-resilience had a moderating effect that emotional labor affected depressive symptom and job satisfaction. @*Conclusion@#In this study, Ego-resilience were found to have a significant moderating effect in relation to emotional labor, depressive symptom, and job satisfaction. Therefore, it's needed to consider the ego-resilience when developing emotional labor management program of nurses.
RESUMO
We attempted to observe the expression of stromelysin, which regulates the extracellular matrix of trabecular meshwork and is one of the family of the matrix metalloproteinases, in the trabecular cells after transfection of replication deficient recombinant adenovirus vector containing stromelysin cDNA. Stromelysin cDNA was produced by RT-PCR with total RNA extracted from cultured human trabecular cells after induction with interleukin-1 alpha, and cloned by inserting the cDNA into the TA vector. Adenovirus vector that contains stromelysin cDNA was constructed by cotransfection of pJM17 and p delta A. CMV. PA-str into the 293 cells. The expression of stromelysin in the trabecular cells was assayed by Western blot and zymography. The sequence of stromelysin cDNA was consistent with that previously reported. The constructed adenovirus vector had stromelysin cDNA but had no E1 region. The expression of stromelysin in the trabecular cells by this vector was detected in 4 days and peaked in 7 days after transfection. In conclusion, this study showed the possibility of gene therapy in the glaucoma treatment by transfecting the trabecular cells with the replication deficient recombinant adenovirus vector containing stromelysin cDNA.