RESUMO
Background: sysmex XT 1800i coulter is a hematology analyzer incorporating new electronic and mechanical design with advanced algorithm technology to perform CBC. The principles of sysmex XT1800 are electrical impedance technology, optical scatter technology, flow cytometry. The sysmex XT-1800i haematology analyser is used in unique fluorescence flow cytometry [FFC] technology. FFC looks at deoxyribonucleic acid [DNA] content, cell size and inner cell complexity rather than cell size alone. This generates remarkably accurate results. Review rates and turnaround time are reduced due to specific fluorochrome labelling. The XT-1800i offers true quantitative immunoglobulin [IG] counting instead of the flagging delivered by other technologies
Patiant and Method: evaluation of this instrument was performed on 200 samples of inpatient and outpatient people in Ain Shams University Hospitals. We collected samples from all departments of the hospital randomly of any age and sex except those of age less than 18 years old
Results: this study was centered upon RBCs indices which were mean corpuscular volume [MCV], mean corpuscular hemoglobin [MCH], red cell distribution width [RDW] after comparing sysmex XT 1800 coulter results for RBCs parameters with morphology by smear review under the microscope. We found that the device sensitivity was 74.7%, 75 %, 83 % and specificity was 74.3%, 79.5 %, and 78.7% according to RBCs size, chromasia and anaesocytosis respectively
Conclusion: at the end of our study we reached to specific criteria at which we must do a blood film smear review to evaluate RBCs abnormalities which cannot be evaluated by the device
RESUMO
Background: obesity and iron deficiency anemia are major health problems that are increasing in Egypt especially in females
Aim of work: this study aimed to evaluate the iron status in obese Egyptian females in comparison to normal weight females
Patients and Methods: forty four obese adult female patients and 44 normal weight healthy females as control group were included in this study. They were all tested for iron profile and CRP using semi quantitative rapid latex agglutination test
Results: the patient group in this study showed a significant lower serum Fe and TSI than the control group, while ferritin was higher in patients than the control group. The comparison between the three groups of obesity showed that the grade III patients had the lowest median value in serum iron and the highest median value in TIBC and ferritin, however no statistical significant differences were detected between the three groups of patients [P>0.05]. Our results showed that 70.4%] of patients had positive CRP with positive correlation between CRP and BMI
Conclusion and Recommendations: obesity is a low inflammatory disease which affects iron profile and increases CRP. Further, study on larger number of cases is recommended to analyze the exact mechanism of iron deficiency anemia in obese female patients
RESUMO
The intravascular and intra-alveolar deposition of fibrin in severe neonatal respiratory distress syndrome [RDS] have been attributed to activation of clotting. We questioned whether in face of enhanced clotting, fibrinolysis is sufficient in these neonates. Therefore, we aimed to assess plasminogen activator inhibitor-1 [PAI-1] as a marker of fibrinolysis in plasma of neonates with RDS to investigate its relation to disease severity and the possible prognostic value of its early measurement. The study included 65 neonates, all of them were clinically assessed within 6 hours of birth for inclusion in the study. The study group consisted of 45 preterm neonates, 30 with RDS, and 15 healthy preterm control neonates. The remaining twenty neonates were fullterms; 10 had clinical evidence of infection and 10 were healthy fullterm control neonates. Neonates with RDS were clinically and radiologically evaluated to assign severity and accordingly they were categorized into a severe group and a mild to moderate group. Blood samples were obtained from these neonates while on mechanical ventilation during the first day of life. Fraction of inspiratory oxygen [FiO2] was determined and they were clinically followed up throughout the whole duration of ventilation. Laboratory investigations included CBC, C-reactive protein [CRP], ABG and plasma PAI-1 determination for all neonates as well as a coagulation assay including PT, PTT, fibrinogen and fibrin degradation products [FDPS] done for 18 RDS neonates. Mean plasma concentrations of PAI-1 were significantly elevated in PT neonates with RDS as a whole [P<0.001] and in each subgroup [P<0.01] as compared to control PT neonates. Severe RDS group showed significantly higher PAI-1 in plasma as compared to the mild to moderate group [P<0.001]. A significant positive correlation existed between PAI-1 and FiO2 in all neonates with RDS [P<0.01]. Fibrinogen levels were significantly lower in neonates with severe RDS as compared to the mild to moderate group [P<0.05] and they were negatively correlated to plasma PAI-1 in all studied RDS neonates [P<0.05]. The ratio of FDPs between 5 and 20 to FDPs<5 was 9:1 in the mild to moderate RDS group, compared to 1:1 in the severe group. Plasma levels of PAI-1 in full term neonates with systemic infection were significantly higher as compared to healthy fullterms [P<0.05]. Sepsis was documented in 21.05% of deaths among neonates with RDS. Retrospective tracing of CRP in neonates with RDS who died revealed values that were non-significantly higher than those of survivors [P>0.05]. Meanwhile, Plasma PAI-1 levels in deceased were significantly higher than those of survivors [P<0.001]. PAI-1 cut off value of 58 ng/ml was 60% sensitive and 73.68% specific to predict mortality in RDS. In severe RDS, high PAI-1 impairs systemic fibrinolysis which likely facilitates the deleterious effects of early clotting activation, contributing to disease severity and mortality. Plasma level of PAI-1 within 24 hours of birth, though might be influenced by early infection may be a useful predictor of outcome in neonatal RDS