Study on secondary metabolic organ of echinacoside in herbs of Cistanche tubulosa / 中国中药杂志

<p><b>OBJECTIVE</b>It could give some theory support of confirming the secondary metabolism organ and regulation of echinacoside in Cistanche tubulosa by searching parasitic growth of C. tubulosa ahd echinacoside variation in different organs of host and parasite.</p><p><b>METHOD</b>The echinacoside content was analyzed by HPLC. The relationship between dry matter accumulation and echinacoside accumulation of C. tubulosa as the well as root diameter of host were comparatively analyzed.</p><p><b>RESULT</b>With the increase of dry matter accumulation of C. tubulosa, echinacoside accumulation increased significantly, and both of them were in significantly positive correlated with the root diameter of host. Echinacoside content in haustorium phloem was 15.53%, higher than that of haustorium xylem, C. tubulosa plant and other organs.</p><p><b>CONCLUSION</b>Haustorium phloem was probably the secondary metabolism organ of echinacoside in C. tubulosa.</p>

Inoculation experiments of Cistanche tubulosa on 8 introduced Tamarix species / 中国中药杂志

<p><b>OBJECTIVE</b>To analyze the inoculation ratio and echinacoside content of Cistanche tubulosa and provide theoretical basis for Tamarix introduction, resource protection and screening of C. tubulosa.</p><p><b>METHOD</b>8 Tamarix species were introduced in the North China Plain and inoculation of C. tubulosa was conducted on all species. Phenylethanoid glycosides fingerprinting and echinacoside content of C. tubulosa were analyzed by using HPLC.</p><p><b>RESULT</b>The adaptability of 8 Tamarix species were significantly different, phenylethanoid glycosides component of C. tubulosa on T. gansuensis and T. austromongolica were basically identical in contrast to T. chinensis, echinacoside content showed no obvious difference in C. tubulosa plant growing 4 months.</p><p><b>CONCLUSION</b>T. gansuensis and T. Austromongolica are suitable for the host introduction plant of C. tubulosa resource protection and screening in North China Plain.</p>

Studied of dry matter accumulation and echinacoside content of Cistanche tubulosa in Huabei plain / 中国中药杂志

<p><b>OBJECTIVE</b>To give some theory support of Cistanche tubulosa cultivation by searching dry matter accumulation and echinacoside content of C. tubulosa.</p><p><b>METHOD</b>Dry matter accumulation content of C. tubulosa culturing in Huabei plain was analysed in different growth season of C. tubulosa. Echinacoside content was determined by HPLC.</p><p><b>RESULT</b>Dry matter accumulation of C. tubulosa showed "S" variation. Dry matter accumulation increased fastest in September among growing seasons. Dry matter amount was 138.58 g after C. tubulosa grew a year. Dry matter amount decreased significantly along with inoculation time retarded. Echinacoside content was 30.59% when C. tubulosa grew in 5 months, decreased guadully after that, and 9.76% in annual.</p><p><b>CONCLUSION</b>Variation rule of dry matter accumulation and echinacoside content was found in C. tubulosa that grew one year in Huabei plain.</p>

Study on inoculation technology of Cistanche tubulosa in the field / 中国中药杂志

<p><b>OBJECTIVE</b>To increase inoculation rate of Cistanche tubulosa in the field by studying inoculation technologies.</p><p><b>METHOD</b>Root-tube inoculation methed was used on field experiments. Inoculation rate of C. tubulosa was compared to different size seeds and inoculation mediums and inoculation time.</p><p><b>RESULT AND CONCLUSION</b>May is suitable inoculation time. The inoculation rate of C. tubulosa is 92.5% while the seed width is more than 0.7 mm and coarse sand is selected during inoculation period.</p>

Effects of cultivation technique on yield and favonoid content of Chrysanthemum flower (Qiju) grown in Hebei / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of cultivation techniques on the flower yield flavonoid content in Chrysanthemum flower grown in Hebei.</p><p><b>METHOD</b>Studied on flowers yield and three factors (transplanting date and plant density and fertilizer quantity) were examined in field experiment at 4 treatments levels.</p><p><b>RESULT AND CONCLUSION</b>The best results were obtained at following conditions: diammonium phosphate 300 kg x hm(-2) and potassium sulfate 150 kg x hm(-2) fertilized before transplanting, transplanting at the first ten days of May and the spacing 40 cm x 40 cm.</p>

Morphology of seed germination and parasitism in Cistanche tubulosa / 中国中药杂志

<p><b>OBJECTIVE</b>To understand the process of Cistanche tubulosa.</p><p><b>METHOD</b>The process of seed germination and parasitism was observed using stereomicroscope.</p><p><b>RESULT</b>Seedling of C. tubulosa sprouted after forty day without host root's contact in fields, a tube-like-organ formed and grew auger-type from host root, the tuber apex where touches host root swelled and formed haustorium. Haustorium intruded host root epidermis and vascular bundles, and released brown substances. Then, embryo bud with six or more young leaves formed, finally the swelled tuber-like-organ broken and seed coat shed. Due to the parasitism of C. tubulosa, the host root near stem site swelled, but the other part, shrunk and disappered gradually.</p><p><b>CONCLUSION</b>Seed of C. tubulosa could germinate indepently in fields. Tuber-like-organ formatin, haustorium formation and bud formation are key steps of C. tubulosa seedling development.</p>