A LONGITUDINAL STUDY ON THE PHYSICAL GROWTH OF INFANTS DURING THE FIRST SIX MONTHS OF LIFE AND THEIR BREASTMILK, ENERGY AND PROTEIN INTAKES / 营养学报

A longitudinal study was carried out on fifty infants selected from both urban and rural areas of Beijing for their body weights, heights, head and mid-upper arm circumferences, tricep and abdomen skinfold thicknesses, and breatmilk and energy and protein intakes at the 1st, 3rd and 6th month of life. The results revealed that the breastmilk intakes of these infants were ranged from 600-800 g/day during this period of time, the breastmilk intakes of the rural infants were higher significantly than that of the urban infant throughout the study period (P

USING "SILMAG" AS AN ABSORBENT IN DETERMINATION OF RIBOFLAVIN / 营养学报

In this paper we recommended a new method, using "Silmag" as an absorbent for the determination of riboflavin content in foods fluorometrically. The lowest detecting value was 0.006 micrograms. The coefficient of variation of riboflavin in determining milk powder and the seed of Job's tears(Coix La-chrymalobi L.)was 2.1% and 3.3% respectively,and the recovery of added riboflavin in bread was 102.7, 102.3 and 99.7% with an average value of 101.4%; milk powder was 96.0, 103.0 and 104.2% with an average of 101.1%. Both precision and accuracy of this method were satisfactory. In determining riboflavin by fluorometric method, the sample must be treated by enzyme after hydrolysis. The "Silmag" can be reactivated after using.

THE RELATIONSHIP BETWEEN THE DIET AND THE GROWTH OF INFANTS DURING THE WEANING PERIOD (6-18 MONTHS) / 营养学报

The growth curves and diet of 185 babies in rural area near Beijing from 6 months to 18 months old have been studied. Infants were divided into 3 groups according to their diet taken:(1) 89 babies had breast milk only;(2) 66 babies took more than 250g animal milk per day besides breast milk;(3) 30 babies who did not have breast milk and only animal milk was taken. All the babies were gradually transferred to an ordinary cereal based diet during the weaning period. To half of them a soybean and cereal based formula fortified with minerals and vitamins was supplied, which would provide 6.6g protein and 141 kcal and some other nutrients per day.The results showed that the growth curves of body weight and height of children were much better than the data obtained in 1975, but still lagged behind that of the urban children, especially for the body weight. The weight curves of the babies who took animal milk seemed higher than the other groups. Female babies who were fed with breast milk and supplemented with the weaning food have a better weight and height curves than non-supplemented ones, but this was not seen in males. The hemoglobin contents of the babies who took animal milk when they were 18 months old were lower than that of the 6 months old, but there were no differences in the breast milk groups, and the lowering of the hemoglobin level in the animal milk groups could be corrected by adding weaning food in the diet. When the babies were 6 months old, 39.3% of them had a hair zinc content below the lower limit of the normal range, and it became to 47.6% when they were 18 months old. So zinc deficiency of the babies were present in this local area, and it should be fortified into the weaning food.

METHODS OF FLUOROMETRIC DETERMINATION OF TRACE AMOUNT OF SELENIUM IN BIOLOGICAL MATERIALS,WATER AND SOIL 2.DETER MINATION OF SELENIUM IN CEREALS AND VEGETABLES / 营养学报

2,3-Diaminonaphthalene was used for the fluorometric determination of selenium in cereals and vegetables. Nitric-perchloric-sulphuric acids mixture was used for digestion. Coefficient of variation and recovery for cereals were 4-10% and 97.1%, and for vegetables were 4-18% and 97.8% respectively. Addition of hydrochloric acid to the final digests could be omitted for ordinary cereals and soybean, but it was necessary for samples from seleni-ferous area and some vegetables with higher selenium content such as mushrooms.

METHODS OF FLUORIMETRIC DETERMINATION OF TRACE AMOUNT OF SELENIUM IN BIOLOGICAL MATERIALS,WATER AND SOIL 1.DETERMINATION OF SELENIUM IN HAIR,BLOOD AND URINE / 营养学报

A modification of Watkinson's method was used for the flaorimetric determination of selenium in blood, hair, urine and animal tissues with 2,3-Di-aminonaphthalene. A mixture of sulphuric, perchloric acid and sodium molybdate was used for digestion. As little as 3 ng selenium in the sample could be estimated out. Coefficients of variation and recoveries for blood, hair, urine and animal tissues were 3.9, 5.5, 3.3 and 5.6%, and 97.0, 95.0, 97.8 and 99.8% respectively. No significant difference in selenium content estimated was found as graded amounts of samples were taken for analysis, indicating no foreign interference in the extracts. Both precision and accuracy of this method are satisfactory.

PROTEIN REQUIREMENTS OF CHINESE MALE ADULTS / 营养学报

The subjects were all Chinese male adults. The average age, body weight and height were 37.9 (24-44) yr, 64.3 (51.5-74.0) kg and 173 (157-180) cm respectively in the first group, and 30.2 (24-41) yr, 67.7 (56.5-70.0) kg and 171 (170-176) cm respectively in the second group.The subjects in the first group were studied for 70 days for the N balance response at graded protein intakes of 0.42, 0.60, 0.73, 0.90 and 1.05 g/kg /day on Chinese mixed diet, during 5 experimental periods respectively. Each period took ten days preceded by one day of low protein diet, and was followed by three days of free choice diet The sequence of protein intakes during the study was 0.60, 0.73, 0.42, 0.90 and 1.05 g/kg/day. Six subjects in the second group were given a mixed diet ata protein level of 0.93 (0.91-0.94) g/kg/day for three months.In the N balance data of the first group, all subjects were in negative balance at protein intake of 0.42, 0.60 and 0.73 g/kg/day and all in positive N balance at 1.05 g/kg/day. Most of the subjects were in positive N balance at 0.90 g/kg/day. The linear regression analysis of N balance response of individual subject is that the intercept at zero balance of individuals ranged from 129.4-192.5 mg N/kg/day with the mean of the group of 147.7? 18.6mg N/kg/day or 0.92?0.12 g protein/kg/day. The five of the six subjects in the second group were in positive N balance, when on a mixed diet of 0.93 ?0.02 g protein/kg/day for three months.The results obtained indicated the mean protein requirement of these subjects based on the linear regression analysis of N balance response of individuals was 147.7 ? 18.6 mg N/kg/day or 0.92?0.12 g protein/kg/day. If 97.5% is to be covered, the safe level of protein intake should be 1.16 g protein/ kg/day.