RESUMO
Objective To investigate the comparation of sodium valproate concentration in peripheral blood monitoring by fluorescence polarization immunoassay method(FPIA)and high performance liquid chromatography method(HPLC)in epilepsy children.Methods 87 cases of epilepsy children received Sodium valproate treatment in our hospital from February 2014 to June 2016 were selected,fasting venous blood of elbow vein were collected the next morning after last medication, blood concentrations of Sodium valproate in serum samples were detected by FPIA method and HPLC method respectively,the correlation and consistency of results of the two methods were observed and compared.Results The intra day and inter day RSD of Sodium valproate concentration in peripheral blood in epilepsy children detected by FPIA were <5%,the recovery rate was 90%-110%,the precision and accuracy were high;The intra day and inter day RSD of Sodium valproate concentration in peripheral blood in epilepsy children detected by HPLC were <5%,the recovery rate was 90%-110%,the precision and accuracy were high; the linear regression equation between determination value of HPLC method (X) and determination value of FPIA method (Y) was:Y=0.8355X+1.8231,correlation coefficient r=0.914,the detection results were positively related;the Sodium valproate blood concentration detected by FPIA was significantly lower than that detected by HPLC method, the difference was statistically significant (P<0.05);Bland-Altman deviation chart results show that determination of blood drug concentration by HPLC method was higher than that of FPIA method by 7.2μg/mL.Conclusion The precision and accuracy of sodium valproate concentration in peripheral blood monitoring by FPIA method and HPLC method were all high, and the correlation was good, but the detection results of the two methods were significantly different,the detection result of HPLC method was higher than that of FPIA method,need to choose and judge according to the clinical situation.
RESUMO
Objective To investigate the effect of two different detection methods of reagent kits on blood concentration of Tacrolimus in liver transplantation .Methods 81 cases of liver transplantation patients received Tacrolimus testing in Zhoushan Hospital from February 2013 to August 2016 were selected, fasting venous blood of upper limb were collected 12h after treatment, blood concentrations of Tacrolimus in whole blood samples were detected by Enzyme linked immunosorbent assay ( ELISA) kit and Enzyme enhanced immunoassay ( EMIT) kit respectively , the results of the two methods were observed and compared .Results The intra day and inter day precision and accuracy of ELISA method were good , RSD <5%, in the low concentration range , the precision of detection was slightly worse; the intraday and inter day precision and accuracy of ELISA method were good , RSD <5%, in the low concentration range , the precision of detection was slightly worse; the Tacrolimus blood concentration measured by ELISA (5.19 ±0.73) μg/L test kit was significantly lower than that measured by EMIT kit (8.29 ±1.14) μg/L, the difference was statistically significant (P<0.05); correlation coefficient of the detection results of two kinds of reagent kit was r =0.9176, the detection results were positively related; the determination results of ELISA kit ( 1.116 ±0.125 ) μg/L were obviously lower than that of EMIT kit (1.507 ±0.201) μg/L when Tacrolimus blood concentration <2g/L, with significant difference (P<0.05).Conclusion ELISA detection kit and EMIT kit can be used to monitor the blood concentration of Tacrolimus in liver transplantation patients , and the correlation was good, but the blood concentrations of Tacrolimus were significantly different between the two kits , need to establish their own of therapeutic window to judge,the test results can not be replaced with each other .