RESUMO
<p><b>AIM</b>To develop a rapid and sensitive method for monosaccharide composition analysis.</p><p><b>METHODS</b>Glycoprotein was first hydrolyzed to monosaccharides, which were subsequently reacetylated (amino monosaccharides), tagged with 2-aminopyridine and then separated and monitored in selected ion mode by CapGCC-LC/MS. The use of tetradeuterium labeled-aminopyridyl-monosaccharides prepared by tagging monosacahrides with hexadeuterium labeled 2-aminopyridine as internal standards improved the linearity and reproducibility in quantification.</p><p><b>RESULTS</b>This method was successfully applied to monosaccharide composition analysis of model glycoproteins, fetuin and erythropoietin down to 1 pmol monosaccharides.</p><p><b>CONCLUSION</b>This method has been shown to be highly sensitive and is applicable to monosaccharide composition analysis of glycoproteins.</p>