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Introduction: For a successful endodontic treatment, antimicrobial property of endodontic filing is mandatory. Hence, the aimof this in vitro study was to assess the antibacterial efficacy of three endodontic sealers on Enterococcus faecalis, Candidaalbicans, and Staphylococcus aureus by direct contact test (DCT).Materials and Methods: The antimicrobial efficacy of three different sealers, AH Plus, mineral trioxide aggregate (MTA)Fillapex, and Endosequence BC was tested against E. faecalis ATCC 29212, C. albicans ATCC 10231 and S. aureus ATCC25923 by DCT. Freshly mixed sealers were placed in flat bottom test tubes incubated at 37°C. Thereafter, 10 μL of suspensionwas drawn and spread over cultural plates to determine the colony count using a digital colony counter. Readings were takenat 1 h (fresh specimen), then at 24 h (after setting). The results were tabulated and statistical analysis was done using oneway ANOVA and Tukey HSD test.Results: DCT showed a significant decrease in microbial count in AH Plus, MTA Fillapex, and Endoseq BC at both the timeintervals. Group 3 (Endosequence BC) showed minimum microbial count followed by Group 2 (MTA Fillapex) and maximumfor Group 1 (AH Plus) for both the time intervals (1 h and 24 h).Conclusion: Endosequence BC showed maximum antimicrobial efficacy against all the tested microorganisms for both thetime intervals, followed by MTA Fillapex and AH Plus.
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Aims: To compare the apical extrusion of Enterococcus faecalis after instrumentation with three different Ni-Ti rotary instruments- An in vitro study. Settings and Design: In vitro study Methods and Material: Forty freshly extracted mandibular premolars were mounted in bacteria collection apparatus and root canals were contaminated with a suspension of Enterococcus faecalis. The contaminated teeth were divided into 4 groups of 10 teeth each according to rotary system used for instrumentation: Group1: Hyflex files, Group 2: GTX files, Group 3: Protaper files and Group 4: control group (no instrumentation). Bacteria extruded after preparations were collected into vials and microbiological samples were incubated in BHI broth for 24 hrs. The colony forming units were determined for each sample. Statistical Analysis Used: Statistical analysis was done using one way ANOVA followed by post hoc independent “t” test. Results: GTX files extruded least amount of bacteria followed by Hyflex files. Maximum extrusion of E. faecalis was seen in rotary Protaper group. Conclusion: Least amount of extrusion was seen with GTX files followed by Hyflex files and then rotary Protaper system.