Study of normal values in coagulation profile.

The objective of the present work was to study normal values of hemostasis parameters in healthy volunteers using Behring Coagulation Timer (BCT). Plasma was divided into 2 parts fresh plasma and lyophilized plasma. It was found that the mean +/- SD of prothrombin time (PT) and activated partial thromboplastin time (APTT) in fresh plasma (n = 37) were 11.95 + 0.7 and 40.52 +/- 5.30 seconds respectively. The means + SD of coagulation factors I, II, VII, VIII and IX were 2.55 +/- 0.73 g/l (n = 36), 82.28 +/- 10.28 per cent (n = 37), 82.79 +/- 19.36 per cent (n=32), 89.13 +/- 24.17 per cent (n = 37), 94.11 +/- 16.29 per cent (n=31) respectively. The normal ranges (p5-p95) of PT, APTT and coagulation factors I, II, VII, VIII and IX were 10.8-13.3 sec, 31.4-48.0 sec and 1.82-4.65 g/l, 64.83-96.5 per cent, 46.88-113.5 per cent, 52.44-127.61 per cent and 67.87-116.94 per cent respectively. Comparison of PT, APTT between fresh plasma and lyophilized plasma were statistically different (Wilcoxon match-pair signed rank test, p < 0.05), while F I, II, VIII and F.IX in fresh plasma were increased more significantly than lyophilized plasma.

Erythrocyte glucose-6-phosphate dehydrogenase and pyruvate kinase activities in hemoglobin H disease.

Erythrocyte glucose-6-phosphate dehydrogenase (G6PD) and pyruvate kinase (PK) activities were studied in hemoglobin H (HbH) patients by spectrophotometric method, cytochemical method and the methemoglobin reduction (MR) test for the detection of heterozygous G6PD deficiency. G6PD deficiency was found in 7 of 64 cases (10.9%), including 3 cases of genotype alpha 1/alpha 2 and 4 cases of genotype alpha 1/CS. None of the HbH patients was found to be PK-deficient. Spectrophotometrically determined G6PD and PK activities were significantly higher in HbH patients than in normals (p less than 0.001), whereas the MR test yielded a significantly lower percentage of residual methemoglobin in HbH patients than in normals (p less than 0.05). All three methods were efficient in the detection of hemizygous G6PD deficiency in HbH patients, but not in G6PD-deficient females.